Abstract
The alphabeta-tubulin heterodimer has two high affinity guanosine 5'-triphosphate binding sites, so that purified tubulin usually contains two molecules of bound guanosine nucleotide. Half this nucleotide is freely exchangeable with exogenous guanine nucleotide, and its binding site has been readily localized to the beta-subunit. The remaining nonexchangeable guanosine 5'-triphosphate can only be released from tubulin by denaturing the protein. We replaced the exchangeable site nucleotide of tubulin with 2'-deoxyguanosine 5'-diphosphate, exposed the resulting tubulin to ultraviolet light, degraded the protein, and isolated ribose-containing peptide derived from the nonexchangeable site. A large cyanogen bromide peptide was recovered, and its further degradation with endoproteinase Glu-C established that cysteine-295 of alpha-tubulin was the major reactive amino acid cross-linked to guanosine by ultraviolet irradiation.
References
Nov 1, 1977·Cell·B M SpiegelmanM W Kirschner
Sep 19, 1978·Biochemistry·H W Detrich, R C Williams
Sep 21, 1976·Biochemistry·R C WeisenbergP J Dickinson
Oct 13, 1992·Biochemistry·D PandaB Bhattacharyya
Apr 9, 1991·Biochemistry·D L Sackett, R E Lippoldt
Mar 20, 1990·Biochemistry·E Hamel, C M Lin
Jul 25, 1989·Biochemistry·M R Mejillano, R H Himes
Mar 21, 1989·Biochemistry·D L SackettJ Wolff
Nov 4, 1986·Biochemistry·E HamelC M Lin
Apr 20, 1987·FEBS Letters·H SternlichtG W Farr
Oct 9, 1974·Biochimica Et Biophysica Acta·A LeviP Calissano
Jul 1, 1974·Journal of Biochemistry·T Kobayashi
Dec 1, 1968·Biochemistry·R C WeisenbergE W Taylor
Aug 28, 1984·Biochemistry·E Hamel, C M Lin
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Oct 23, 1984·Biochemistry·E HamelC M Lin
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E KrauhsH Ponstingl
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H PonstinglT Kempf
Aug 20, 1993·Science·T J Mitchison
May 15, 1994·European Journal of Biochemistry·S Grover, E Hamel
Feb 1, 1994·Biochemistry·K E ShearwinS N Timasheff
Jan 15, 1998·Nature·E NogalesK H Downing
Citations
Oct 18, 2002·Biopolymers·Ernest Hamel
Dec 23, 2008·Applied Microbiology and Biotechnology·Bon-Sung KooMoon-Young Yoon
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J DayelR D Mullins
Apr 8, 2015·Chemical Biology & Drug Design·Shanthi NagarajanAe Nim Pae
Jul 19, 2002·Biophysical Journal·Ozlem KeskinDavid G Covell
Apr 23, 1999·The Journal of Biological Chemistry·R BaiE Hamel
May 5, 2004·The Journal of Biological Chemistry·Ruoli BaiErnest Hamel
Apr 28, 2009·Plant Physiology and Biochemistry : PPB·Bon-Sung KooMoon-Young Yoon