Direct Plasmon-Enhanced Electrochemistry for Enabling Ultrasensitive and Label-Free Detection of Circulating Tumor Cells in Blood

Analytical Chemistry
Shan-Shan WangChen Wang

Abstract

In this work, we developed a simple electrochemical method for ultrasensitive and label-free detection of circulating tumor cells (CTCs) based on direct plasmon-enhanced electrochemistry (DPEE). After plasmonic gold nanostars (AuNSs) were modified on the glassy carbon (GC) electrode, the aptamer probe was immobilized on the AuNSs surface, which can selectively capture the CTCs in samples. Upon localized surface plasmon resonance (LSPR) excitation, the electrochemical current response can be enhanced remarkably due to efficient hot electrons transport from AuNSs to the external circuit. The captured cells on the AuNSs surface will influence the hot electrons transport efficiency, leading to a decreased current response. Using ascorbic acid (AA) as the electroactive probe, it was found that the current responses of the AuNSs/GC electrode upon light irradiation decrease with the cell concentration. Due to the special molecular recognition of the aptamer and enhanced electrochemical performance of the plasmon, the proposed method enables an ultrasensitive and label-free detection of CTCs with excellent selectivity. The experimental results show that CCRF-CEM cell concentrations as low as 5 cells/mL can be successfully detected, whi...Continue Reading

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Citations

Feb 27, 2020·Thoracic Cancer·Zhenhua ZhangMin Liu
Aug 27, 2019·Frontiers in Chemistry·Noemi BellassaiGiuseppe Spoto
Apr 5, 2020·Mikrochimica Acta·Junjun LuoMinghui Yang
Dec 29, 2019·Scientific Reports·Jason Chia-Hsun HsiehMin-Hsien Wu
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
Aug 8, 2021·International Journal of Molecular Sciences·Han ZhouZhenguo Zhang
Nov 17, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qiuyang XuChen Wang

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