Direct polymerase chain reaction detection of Campylobacter spp. in poultry hatchery samples

Avian Diseases
Kelli L HiettNorman J Stern

Abstract

A rapid, sensitive, and specific polymerase chain reaction (PCR) assay was developed for the direct detection of Campylobacter in environmental samples from hatcheries. PCR, with a set of primers specific for the Campylobacter flaA short variable region (SVR), detected the presence of Campylobacter in both fluff and eggshell samples; however, a determination of whether the organism was living or dead could not be made. Conventional cultural methods detected no Campylobacter from the same samples. An additional benefit of the direct PCR assay is it allows for the production of a product that can be sequenced to provide further epidemiologic information.

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Citations

Nov 27, 2002·Applied and Environmental Microbiology·K L HiettS Ladely
Aug 7, 2003·Applied and Environmental Microbiology·D G Newell, C Fearnley
Sep 8, 2006·Applied and Environmental Microbiology·Kenneth A CallicottNorman J Stern
Apr 8, 2009·Acta Veterinaria Scandinavica·Päivikki Perko-MäkeläUlrike Lyhs
Apr 1, 2003·Animal Health Research Reviews·Orhan SahinQijing Zhang
Dec 3, 2015·Poultry Science·S Ingresa-CapaccioniC Marin
Nov 25, 2003·Journal of Applied Microbiology·O SahinQ Zhang
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Oct 17, 2015·Avian Diseases·Orhan SahinQijing Zhang
Jan 8, 2004·Avian Diseases·Richard L ZiprinRoger B Harvey
Apr 30, 2020·Animals : an Open Access Journal From MDPI·Beata WysokJoanna Szteyn

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Methods Mentioned

BETA
PCR
PCRs
reverse transcription PCR

Software Mentioned

ClustalX
PAUP
Sequencher

Related Concepts

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Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.