PMID: 8945890Nov 1, 1996Paper

Direct preconditioning of cardiac ventricular myocytes via adenosine A1 receptor and KATP channel

The American Journal of Physiology
B T Liang

Abstract

Both adenosine receptor and ATP-sensitive K (KATP) channel mediate the protective effect of ischemic preconditioning in the intact heart. The objective of the present study was to determine the role of adenosine receptor and KATP channel as well as their interaction in simulating and mediating preconditioning of the cardiac myocyte. Cardiac ventricular myocytes cultured from chick embryos 14 days in ovo were developed as a myocyte model of preconditioning. Myocytes were preconditioned by exposing them to 5-min hypoxia, termed preconditioning hypoxia, before a second 90-min hypoxia. Preconditioning resulted in a 64 +/- 3% decrease in the amount of creatine kinase released and a 66 +/- 2% reduction in the percentage of myocytes (+/-SE, n = 11) killed. Glibenclamide or 5-hydroxydecanoic acid (5-HD), when present during the preconditioning hypoxia, blocked the preconditioning effect. Prior exposure of the myocytes to pinacidil also led to a decrease in the injury sustained during the 90-min hypoxia. The protective effect of pinacidil was blocked by glibenclamide or 5-HD, suggesting that KATP channel activation can mimic as well as mediate preconditioning. Adenosine receptor antagonist 8-sulfophenyltheophylline (8-SPT) blocked the p...Continue Reading

Citations

Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·Q Zhang, Z Yao
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J HuhB T Liang
Mar 15, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jason N Peart, Garrett J Gross
May 6, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Zhiping CaoDonna M Van Winkle
Dec 28, 2010·Canadian Journal of Physiology and Pharmacology·Laurice Fretwell, John M Dickenson
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J P HeadrickG P Matherne
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Huiping LiuZhenhai Yao
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T S MonahanJ G Dobson
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jennifer M GordonRebecca H Ritchie

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