Direct purification of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography without presaturation of the two-phase solvent mixture

Journal of Separation Science
Dingfang WuShihua Wu

Abstract

Tanshen, the rhizome of Salvia miltiorrhiza Bunge, is a famous Traditional Chinese Medicine for multiple therapeutic remedies. This work presents the isolation and purification of tanshinone I and tanshinone IIA from the extract of the rhizome of S. miltiorrhiza by using high-speed counter-current chromatography (CCC) without presaturation of the two-phase solvent mixture. The CCC method combines the results of CCC solvent system selection and components analyses of solvent mixture by GC, and thus it is possible to add accurately each individual solvent to prepare single saturated solvent phase without presaturation. The optimum CCC solvent system is a system of hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v), which has been determined by usual solvent system selection and CCC runs. As a result, over 98% pure tanshinone IIA and over 94% pure tanshinone I have been obtained by using less solvent volume. Their structures have been identified by ESI-MS, NMR spectra.

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Citations

Jun 6, 2014·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Krystyna Skalicka-Woźniak, Ian Garrard
Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Dec 25, 2012·Analytical and Bioanalytical Chemistry·Omer ShehzadYeong Shik Kim
Oct 14, 2019·Journal of Chromatography. a·Lihong Zhang, Shihua Wu

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