Direct quantification of norepinephrine spillover and hormone output from the pancreas of the conscious dog

The American Journal of Physiology
B E DunningA D Cherrington

Abstract

To estimate pancreatic neural activity and to assess the potential role of the pancreatic nerves in the regulation of hormone secretion, the methodology necessary to quantify neurotransmitter spillover and hormone output in the conscious dog was developed. A femoral artery and the superior pancreaticoduodenal vein (SPDV) were chronically cannulated, and a flow probe was placed on the SPDV. Hormone output was calculated using the pancreatic arteriovenous concentration difference and the SPDV plasma flow. Basal glucose levels were 103 +/- 1 mg/dl; the pancreatic outputs of insulin, glucagon, and pancreatic polypeptide (PP, an index of parasympathetic neural activity) were 2,900 +/- 700 microU/min, 1,900 +/- 400 pg/min, and 9.3 +/- 4.6 ng/min, respectively. Pancreatic norepinephrine (NE) spillover was calculated similarly; however, pancreatic extraction of epinephrine was used as an index of NE extraction. Basal NE spillover was 3,600 +/- 700 pg/min, greatly exceeding that reported using anesthetized, laparotomized dogs. Intravenous glucose infusion increased plasma glucose to 146 +/- 13 mg/dl, increased insulin output approximately twofold, and suppressed glucagon output by approximately 50%. Hyperglycemia markedly reduced PP out...Continue Reading

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Citations

Jun 4, 2015·American Journal of Physiology. Endocrinology and Metabolism·Thomas O MundingerGerald J Taborsky
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·L BenthemG J Taborsky
Jul 24, 2003·American Journal of Physiology. Endocrinology and Metabolism·Thomas O MundingerGerald J Taborsky
Jun 18, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Marcia R BatistaMary Courtney Moore
Sep 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·Q MeiG J Taborsky
Mar 25, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kathryn M CanniffMary Courtney Moore
Apr 27, 2021·American Journal of Physiology. Endocrinology and Metabolism·Chien-Chia ChenShiue-Cheng Tang

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