Direct quantification of the modulation of interaction between cell- or surface-bound LFA-1 and ICAM-1

Journal of Leukocyte Biology
Joana VitteP Bongrand

Abstract

The functional activity of leukocyte integrins is highly regulated by several mechanisms related to intrinsic molecular properties and receptor interaction with the cell membrane. Here, we present a microkinetic study of the lymphocyte function-associated antigen-1-mediated interaction between flowing Jurkat cells and surface- or cell-bound intercellular adhesion molecule-1 (ICAM-1). We conclude that adhesion is initiated by the formation of a single bond with approximately 0.3 s(-1) dissociation rate, and attachment is subsequently strengthened by the formation of additional bonds during the next 10 s; exposing cells to Mg2+ or Mn2+ resulted in up to a 16-fold increase of the binding frequency, in line with reported measurements performed on isolated molecules with surface plasmon resonance methodology; cell-bound ICAM-1 molecules were more efficient in mediating adhesion than Fc-ICAM-1, properly oriented and bound by surface-adsorbed protein A; and quantitative analysis of binding frequency suggested that adhesion efficiency was ten- to 100-fold lower than the maximum value allowed by previously determined association rates of soluble molecules. It is concluded that the presented methodology provides a simple and unique way o...Continue Reading

References

Jan 1, 1992·Biophysical Journal·O TissotP Bongrand
May 1, 1983·European Journal of Immunology·P BongrandP Golstein
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D DavignonT A Springer
Nov 3, 1995·The Journal of Biological Chemistry·A PierresP Bongrand
Mar 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S L ErlandsenR D Nelson
Jun 16, 1995·The Journal of Biological Chemistry·T Q Cai, S D Wright
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Foote, C Milstein
Apr 15, 1994·Science·E L FlorinH E Gaub
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·A PierresP A van der Merwe
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·A PierresP Bongrand
Sep 9, 2000·Current Opinion in Cell Biology·Y van Kooyk, C G Figdor
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Chen, T A Springer
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·E Evans
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·M ShimaokaT A Springer
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M L LupherD E Staunton
May 23, 2002·Biophysical Journal·Anne PierresPierre Bongrand

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Citations

Dec 1, 2008·Cellular and Molecular Bioengineering·Anne PierresPierre Bongrand
Jul 1, 2009·The Journal of Cell Biology·Fang KongCheng Zhu
Jun 3, 2009·Biophysical Journal·Philippe RobertPierre Bongrand
Aug 29, 2007·Journal of Molecular Recognition : JMR·Philippe RobertPierre Bongrand

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