Direct quantitative bioanalysis of drugs in dried blood spot samples using a thin-layer chromatography mass spectrometer interface

Analytical Chemistry
Paul Abu-Rabie, Neil Spooner

Abstract

The CAMAG thin-layer chromatography mass spectrometer (TLC-MS) interface has been assessed as a tool for the direct quantitative bioanalysis of drugs from dried blood spot (DBS) samples, using an MS detector, with or without high-performance liquid chromatography (HPLC) separation. The approach gave acceptable sensitivity, linearity, accuracy, and precision data for bioanalytical validations with and without the inclusion of HPLC separation. In addition, the direct elution technique was shown to increase assay sensitivity for a range of analytes representing a wide "chemical space" for pharmaceutical-type molecules over that obtained by conventional manual extraction of samples (punching of DBS and elution with solvent prior to HPLC-MS analysis). Investigations were performed to optimize extraction time, minimize sample-to-sample carry-over, and compare chromatographic performance. On the basis of this preliminary assessment, it has been demonstrated that the TLC-MS interface has the potential to be an effective tool for the direct analysis of drugs in DBS samples at physiologically relevant concentrations, an approach that could provide significant time and cost savings and greatly simplify bioanalytical procedures compared to...Continue Reading

References

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Citations

Jun 28, 2011·Analytical and Bioanalytical Chemistry·Julien DéglonChristian Staub
Feb 24, 2012·Analytical and Bioanalytical Chemistry·Elena S ChernetsovaGertrud E Morlock
Nov 23, 2012·Analytical Chemistry·Plamen A Demirev
Aug 27, 2011·Lab on a Chip·Mais J JebrailAaron R Wheeler
Nov 19, 2010·Bioanalysis·Gary Emmons, Malcom Rowland
Feb 23, 2011·Bioanalysis·Joanne MatherRobert S Plumb
Oct 21, 2011·Bioanalysis·Fumin LiSteve Michael
Oct 21, 2011·Bioanalysis·Yifan Shi, Hongliang Jiang
Sep 6, 2012·Bioanalysis·Qin C JiMark E Arnold
Sep 11, 2014·Clinical Pharmacokinetics·Abraham J WilhelmEleonora L Swart
Apr 5, 2014·Expert Review of Proteomics·Ramisetti Nageswara Rao
Apr 24, 2012·Journal of Pharmaceutical and Biomedical Analysis·Rayane MohamedMathieu Peyrou
Jan 10, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Norbert GanzMirko Glinski
Sep 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Julien DéglonChristian Staub

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