PMID: 6153208Jan 1, 1980Paper

Direct radioimmunoassay for the detection of IgM antibodies against Mycoplasma pneumoniae

Journal of Immunological Methods
P C Price

Abstract

A direct solid-phase radioimmunoassay is described for detecting IgM-class antibodies against Mycoplasma pneumoniae. The assay achieves a prelminary separation of IgM from other serum proteins by immunoadsorption to anti-IgM-coated wells in microtitre plates. The IgM is then tested for antigen specificity by measuring its ability to bind radiolabelled M. pneunoiae. Positive results are confirmed by retesting sera after treatment with 2-metcaptoethanol. The assay is specific for IgM-class antibodies and specific for M. pneumoniae. It is not affected by competitive inhibition from IgG and avoids the use of density gradient centrifugation or gel filtration to separate IgM from other immunoglobulins. It is sensitive, reproducible, rapid, simple and requires very little serum.

References

May 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·H Brunner, R M CHANOCK

Citations

Jun 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L HirschbergT Vikerfors
Apr 1, 1984·European Journal of Clinical Microbiology·A J van GriethuysenA M van Loon
Mar 1, 1986·Diagnostic Microbiology and Infectious Disease·R Kohler
Jan 1, 1988·Scandinavian Journal of Infectious Diseases·T VikerforsC A Pettersson
Feb 1, 1988·Epidemiology and Infection·R R CoombsT G Wreghitt
Apr 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·M Vejtorp

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