Direct role of NF-kappaB activation in Toll-like receptor-triggered HLA-DRA expression

European Journal of Immunology
Keun-Wook LeeHyung-Joo Kwon

Abstract

Microbial components, such as DNA containing immunostimulatory CpG motifs (CpG-DNA) and lipopolysaccharides (LPS), elicit the cell surface expression of MHC class II (MHC-II) through Toll-like receptor (TLR)/IL-1R. Here, we show that CpG-DNA and LPS induce expression of the HLA-DRA in the human B cell line, RPMI 8226. Ectopic expression of the dominant negative mutant of CIITA and RNA interference targeting the CIITA gene indicate that CIITA activation is not enough for the maximal MHC-II expression induced by CpG-DNA and LPS. Additionally, nuclear factor (NF)-kappaB activation is required for the CpG-DNA-activated and LPS-activated HLA-DRA expression, whereas IFN-gamma-induced MHC-II expression depends on CIITA rather than on NF-kappaB. Comprehensive mutant analyses, electrophoretic mobility shift assays and chromatin immunoprecipitation assays, reveal that the functional interaction of NF-kappaB with the promoter element is necessary for the TLR-mediated HLA-DRA induction by CpG-DNA and LPS. This novel mechanism provides the regulation of MHC-II gene expression with complexity and functional diversity.

References

Jan 1, 1995·Annual Review of Biochemistry·C Schindler, J E Darnell
Apr 6, 1995·Nature·A M KriegD M Klinman
Jan 1, 1996·Annual Review of Immunology·B MachW Reith
Feb 1, 1997·Methods : a Companion to Methods in Enzymology·V OrlandoR Paro
Sep 12, 1997·Science·J E Darnell
Jun 5, 1998·Trends in Biochemical Sciences·S N Maity, B de Crombrugghe
Feb 19, 2000·Current Opinion in Immunology·K V Anderson
Aug 11, 2000·Molecular and Cellular Biology·J A Harton, J P Ting
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D AskewC V Harding
Dec 29, 2000·Nature·H HemmiS Akira
Jun 29, 2001·Nature Immunology·G W Beresford, J M Boss
Feb 28, 2002·Annual Review of Immunology·Arthur M Krieg
Apr 4, 2002·Trends in Immunology·Chilakamarti V RamanaGeorge R Stark
May 2, 2002·Cell·Jenny Pan-Yun Ting, John Trowsdale
Dec 7, 2002·Current Topics in Microbiology and Immunology·G M Barton, R Medzhitov
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aparna RavalDinah S Singer
Jun 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rish K PaiClifford V Harding
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John KuchteyClifford V Harding
Oct 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Peter J van den ElsenJeremy M Boss
Oct 16, 2004·Molecular and Cellular Biology·Hyung-Joo KwonMaureen A Harrington

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Citations

Apr 5, 2012·The Journal of Experimental Medicine·Keunwook LeeMark Boothby
Sep 8, 2009·Biochemical and Biophysical Research Communications·Su Ho HanHyung-Joo Kwon
Apr 2, 2010·BMB Reports·Byoung Kwon ParkHyung Joo Kwon
Dec 24, 2008·Biochemical and Biophysical Research Communications·Dongbum KimHyung-Joo Kwon
Nov 15, 2017·Immunologic Research·Julie HervéBlandine Lieubeau
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cristina CasalsAntonio Celada

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