Direct saponification preparation and analysis of free and conjugated phytosterols in sugarcane (Saccharum officinarum L.) by reversed-phase high-performance liquid chromatography

Food Chemistry
Simin FengKaichen Tang

Abstract

A simple method based on direct saponification followed by RP-HPLC analysis was developed for quantification of free and conjugated sterols in sugarcane. Acid hydrolysis prior to alkaline saponification was used to determined acylated steryl glycoside and steryl glycoside in sugarcane. The applicability and generality of this method were improved with intensive investigation. Compared to traditional solvent extraction method, this method was more time saving and appropriate for characterization of sterol fractions in sugarcane. This method was successfully applied for determination of free and conjugated sterols in different sugarcane samples. The results exhibited that stigmasterol (varied from 883.3 ± 23.5 to 1823.9 ± 24.5 μg/g dry weigh) and β-sitosterol (varied from 117.6 ± 19.9 to 801.4 ± 33.5 μg/g dry weight) were major phytosterols in the sugarcane sample, and their glycosylated forms accounted for almost 87.0% of stigmasterol and 87.5% of β-sitosterol in sugarcane, respectively. In addition, among other parts of sugarcane, tips contained the greatest amount of phytosterols.

References

Jan 5, 2002·Journal of Chromatography. a·S L Abidi
Sep 25, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Patrick BreinhölderWolfgang Lindner
Nov 9, 2002·Phytochemistry·Gladys NuissierMarie Hélène Lescure
Apr 20, 2006·Journal of Pharmaceutical and Biomedical Analysis·M J LagardaR Farré
Jun 27, 2006·Steroids·Patricia GeorgesPaul Bourgeois
Feb 28, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaobo LinRichard E Ostlund
Jan 14, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Franca Marangoni, Andrea Poli
Jul 28, 2012·The American Journal of Clinical Nutrition·Alexander D MirasCarel W le Roux
Dec 25, 2012·Chemistry and Physics of Lipids·Songbai Liu, Huina Ruan
Jun 8, 2013·Journal of Agricultural and Food Chemistry·Xu ZhaoSung Hoon Kim

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Citations

May 10, 2016·Journal of Separation Science·Lei-Wen XiangJin-Ming Lin
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Herminia López-SalazarAntonio R Jiménez-Aparicio
Mar 28, 2018·Journal of Agricultural and Food Chemistry·Simin FengZisheng Luo

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