Abstract
A simple method based on direct saponification followed by RP-HPLC analysis was developed for quantification of free and conjugated sterols in sugarcane. Acid hydrolysis prior to alkaline saponification was used to determined acylated steryl glycoside and steryl glycoside in sugarcane. The applicability and generality of this method were improved with intensive investigation. Compared to traditional solvent extraction method, this method was more time saving and appropriate for characterization of sterol fractions in sugarcane. This method was successfully applied for determination of free and conjugated sterols in different sugarcane samples. The results exhibited that stigmasterol (varied from 883.3 ± 23.5 to 1823.9 ± 24.5 μg/g dry weigh) and β-sitosterol (varied from 117.6 ± 19.9 to 801.4 ± 33.5 μg/g dry weight) were major phytosterols in the sugarcane sample, and their glycosylated forms accounted for almost 87.0% of stigmasterol and 87.5% of β-sitosterol in sugarcane, respectively. In addition, among other parts of sugarcane, tips contained the greatest amount of phytosterols.
References
Jan 5, 2002·Journal of Chromatography. a·S L Abidi
Aug 10, 2002·Progress in Lipid Research·Robert A MoreauKevin B Hicks
Sep 25, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Patrick BreinhölderWolfgang Lindner
Nov 9, 2002·Phytochemistry·Gladys NuissierMarie Hélène Lescure
Feb 21, 2006·Journal of Pharmaceutical and Biomedical Analysis·V D P NairJ Hoogmartens
Apr 20, 2006·Journal of Pharmaceutical and Biomedical Analysis·M J LagardaR Farré
Jun 27, 2006·Steroids·Patricia GeorgesPaul Bourgeois
Jul 13, 2007·Journal of Chromatography. a·Wen-Hui LiuShao-Min Liu
Feb 28, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaobo LinRichard E Ostlund
Jan 2, 2010·Journal of Chromatography. a·M Orozco-SolanoM D Luque de Castro
Jan 14, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Franca Marangoni, Andrea Poli
May 4, 2011·Lipids·Xiaobo LinRichard E Ostlund
Jul 28, 2012·The American Journal of Clinical Nutrition·Alexander D MirasCarel W le Roux
Sep 18, 2012·Food Chemistry·Margaret Slavin, Liangli Lucy Yu
Dec 25, 2012·Chemistry and Physics of Lipids·Songbai Liu, Huina Ruan
Jun 8, 2013·Journal of Agricultural and Food Chemistry·Xu ZhaoSung Hoon Kim
Jan 15, 2014·Journal of Pharmaceutical and Biomedical Analysis·Xinxin ZhangYoichiro Ito
Jan 16, 2014·Food Chemistry·Simin FengBaiyi Lu
Apr 16, 2014·Food Chemistry·Ho-Soo LimMeehye Kim
Citations
May 10, 2016·Journal of Separation Science·Lei-Wen XiangJin-Ming Lin
Oct 17, 2017·Food & Function·Simin FengChung S Yang
Nov 22, 2017·Archives of Physiology and Biochemistry·Khaled AthmouniHabib Ayadi
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Herminia López-SalazarAntonio R Jiménez-Aparicio
Sep 1, 2017·Biomedical Chromatography : BMC·Ana NovakPilar Pérez-Lozano
Aug 2, 2019·Oecologia·Maxime R Hervé, Matthias Erb
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Simin FengZisheng Luo
Feb 3, 2020·Food Chemistry·Simin FengZisheng Luo
Mar 28, 2018·Journal of Agricultural and Food Chemistry·Simin FengZisheng Luo