Direct stimulation of phosphatidylinositol degradation by addition of vasopressin to purified rat liver plasma membranes

M A WallaceJ N Fain


Rat liver plasma membranes were incubated in Ca2+-free buffer plus 0.5 mM ethyleneglycol-bis(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) containing 0.5 mg/ml deoxycholate or 50 mU/ml vasopressin or both compounds. The membrane phospholipids were extracted, separated by two-dimensional chromatography and quantitated by phosphorus analysis. Vasopressin significantly enhanced degradation of phosphatidylinositol in the presence of deoxycholate (21% with deoxycholate alone versus 32% in the presence of vasopressin plus deoxycholate). However, no significant effects of deoxycholate or vasopressin were seen on the degradation of phosphatidylcholine, phosphatidylethanolamine or phosphatidylserine. These results indicate that specific degradation of phosphatidylinositol can be seen after direct addition of vasopressin to a plasma membrane preparation.


Jan 1, 1986·The Journal of Membrane Biology·M C Sekar, L E Hokin
May 2, 1983·Life Sciences·J N FainM A Wallace
Nov 1, 1984·Biological Reviews of the Cambridge Philosophical Society·P H ReinhartF L Bygrave
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J H Exton

Related Concepts

Plasma Membrane
Deoxycholic Acid, Sodium Salt, 12beta-Isomer
Membrane Lipids

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