PMID: 9440513Jan 24, 1998Paper

Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri

Journal of Bacteriology
J BelfaizaI Saint Girons

Abstract

A gene library of the Leptospira meyeri serovar semaranga strain Veldrat S.173 DNA has been constructed in a mobilizable cosmid with inserts of up to 40 kb. It was demonstrated that a Leptospira DNA fragment carrying metY complemented Escherichia coli strains carrying mutations in metB. The latter gene encodes cystathionine gamma-synthase, an enzyme which catalyzes the second step of the methionine biosynthetic pathway. The metY gene is 1,304 bp long and encodes a 443-amino-acid protein with a molecular mass of 45 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The deduced amino acid sequence of the Leptospira metY product has a high degree of similarity to those of O-acetylhomoserine sulfhydrylases from Aspergillus nidulans and Saccharomyces cerevisiae. A lower degree of sequence similarity was also found with bacterial cystathionine gamma-synthase. The L. meyeri metY gene was overexpressed under the control of the T7 promoter. MetY exhibits an O-acetylhomoserine sulfhydrylase activity. Genetic, enzymatic, and physiological studies reveal that the transsulfuration pathway via cystathionine does not exist in L. meyeri, in contrast to the situation found for fungi and some bacteria. Our results in...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jan 1, 1987·Methods in Enzymology·K Soda
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J BelfaizaI Saint-Girons
Jan 1, 1987·Methods in Enzymology·P C Jocelyn
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jul 1, 1967·Journal of Bacteriology·R C Johnson, V G Harris
Jul 21, 1967·Biochemical and Biophysical Research Communications·H Robichon-Szulmajster, H Cherest

❮ Previous
Next ❯

Citations

Mar 18, 2000·Infection and Immunity·E PradelC Locht
Oct 12, 2000·FEMS Microbiology Letters·P Bourhy, I Saint Girons
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·A BrenotR Zuerner
Sep 11, 1999·Journal of Bacteriology·P Vermeij, M A Kertesz
May 3, 2003·FEMS Microbiology Letters·Yasuo YoshidaYoshio Nakano
Dec 19, 2020·Protein Expression and Purification·Vitalia V KulikovaTatyana V Demidkina
Sep 3, 2003·FEMS Microbiology Letters·Mathieu PicardeauIsabelle Saint Girons
Dec 22, 2004·Biotechnology Advances·Dharmendra Kumar, James Gomes
Dec 18, 2010·Journal of the American Chemical Society·Kalyanaraman Krishnamoorthy, Tadhg P Begley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.