PMID: 11607196Jul 1, 1991Paper

Direct transport of ADPglucose by an adenylate translocator is linked to starch biosynthesis in amyloplasts

Proceedings of the National Academy of Sciences of the United States of America
J Pozueta-RomeroT Akazawa

Abstract

Starch biosynthesis has been studied by using amyloplasts isolated from cultured cells of sycamore trees (Acer pseudoplatanus L.). Highly purified intact amyloplasts, free from mitochondria and starch granules derived from broken amyloplasts, were isolated from a Percoll step gradient. Subsequently, the double silicone oil layer centrifugation technique was used to study adenylate transport in the amyloplasts. An adenylate-specific carrier was found to be active in the uptake of ATP, ADP, AMP, and most importantly, ADPglucose (ADP-Glc). Kinetic analyses showed that the uptake of these adenylates was mutually competitive with each other. In contrast to the mitochondrial adenylate carrier, in amyloplasts only ATP and ADP-Glc uptake were inhibited by carboxyatractyloside. Evidence is presented that the ADP-Glc transported into the amyloplast stroma can be used in starch synthesis catalyzed by starch synthase (ADP-Glc:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase, EC 2.4.1.21). We propose that starch biosynthesis in amyloplasts is tightly coupled with the direct transport of ADP-Glc synthesized in the cytosol by sucrose synthase (ADP-Glc:D-fructose 2-alpha-D-glucosyltransferase, EC 2.4.1.13)

References

Dec 1, 1976·Biochemical Genetics·P S Chourey, O E Nelson
Nov 27, 1985·Biochemical and Biophysical Research Communications·D MacherelT Kuroiwa
Oct 17, 1968·European Journal of Biochemistry·E Pfaff, M Klingenberg
Mar 1, 1988·Plant Physiology·E EcheverriaJ C Shannon
Mar 1, 1990·Plant Physiology·P A LizotteS H Duke

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Citations

Feb 1, 1994·Photosynthesis Research·T Akazawa
Dec 30, 1994·Biochimica Et Biophysica Acta·N E Hynes, D F Stern
Mar 6, 1999·Current Opinion in Plant Biology·U I Flügge
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Edurne Baroja-FernándezJavier Pozueta-Romero
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·H. E. Neuhaus, M. J. Emes
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Rodriguez-LópezJ Pozueta-Romero
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth GoutRichard Bligny
Jul 6, 2013·Biotechnology Advances·Abdellatif BahajiJavier Pozueta-Romero
Apr 28, 2004·FEBS Letters·Edmund R S Kunji
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Siv G E AnderssonCharles G Kurland
Jan 26, 2006·Plant & Cell Physiology·Edurne Baroja-FernandezJavier Pozueta-Romero
Jun 14, 2005·Plant & Cell Physiology·Francisco José MuñozJavier Pozueta-Romero
May 25, 1994·Molecular & General Genetics : MGG·M J Giroux, L C Hannah

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