Direct UV determination of Amadori compounds using ligand-exchange and sweeping capillary electrophoresis

Analytical and Bioanalytical Chemistry
Jiahao YuLianfu Zhang

Abstract

The detection of Amadori compounds poorly absorbing in the UV region is challenging. To cope with this problem, a simple and effective method using ligand-exchange and sweeping capillary electrophoresis was first developed. With this method, Amadori compounds can be detected by UV at 236 nm without derivatization steps and separated based on the stable levels of Amadori compound-Cu(2+) complexes. Under optimal conditions with background electrolyte of 50 mmol/L copper sulfate, pH 3.94, and separation voltage 25 kV, baseline separations of the following five Amadori compounds, N-(1-deoxy-D-fructos-1-yl) valine (Fru-Val), N-(1-deoxy-D-fructos-1-yl) leucine (Fru-Leu), N-(1-deoxy-D-fructos-1-yl) alanine (Fru-Ala), N-(1-deoxy-D-fructos-1-yl) methionine (Fru-Met), N-(1-deoxy-D-fructos-1-yl) glutamic acid (Fru-Glu), were achieved within a 20 min run. The method was applied to analyze these Amadori compounds in two tomato products. Fru-Glu could be quantitated sensitively down to 0.706 mg/L in MS powder (a kind of tomato powder), 0.726 mg/L in MS liquid (a kind of concentrated tomato juice), and the limit of quantification for the others ranged from 0.792 to 1.084 mg/L. The most abundant Amadori compound was Fru-Glu (1057.5 mg/100 g) i...Continue Reading

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Citations

Aug 4, 2018·Electrophoresis·Andrea ŠlampováPetr Gebauer
May 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Binh Thanh Nguyen, Min-Jung Kang
Aug 2, 2017·Electrophoresis·Alain Wuethrich, Joselito P Quirino
Aug 15, 2017·Electrophoresis·Véréna PoinsotFrançois Couderc
Jan 4, 2018·Analytical Chemistry·Robert L C VoetenGovert W Somsen

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