Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Einar E ErikssonL Lindbom

Abstract

Leukocyte infiltration in atherosclerosis has been extensively investigated by using histological techniques on fixed tissues. In this study, intravital microscopic observations of leukocyte recruitment in the aorta of atherosclerotic mice were performed. Interactions between leukocytes and atherosclerotic endothelium were highly transient, thereby limiting the ability for rolling leukocytes to firmly adhere. Leukocyte rolling was abolished by function inhibition of P-selectin (P<0.001, n=8), whereas antibody blockage of E-selectin (n=10) decreased rolling leukocyte flux to 51 +/- 9.9% (mean+/-SE, P<0.01) and increased leukocyte rolling velocity to 162 +/- 18% (P<0.01) of pretreatment values. Notably, function inhibition of the integrin alpha(4) subunit (n=5) had no effect on rolling flux (107+/-25%, P=0.782) or rolling velocity (89+/-6.1%, P=0.147), despite endothelial expression of vascular cell adhesion molecule 1 (VCAM-1). Leukocytes interacting with atherosclerotic endothelium were predominantly neutrophils, because treatment with antineutrophil serum decreased rolling and neutrophil counts in peripheral blood to the same extent. In conclusion, we present the first direct observations of atherosclerosis in vivo. We show th...Continue Reading

References

May 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·G K HanssonG Bondjers
Feb 1, 1995·The Journal of Experimental Medicine·K LeyA L Beaudet
Jan 1, 1995·Journal of Leukocyte Biology·M K SquierJ J Cohen
Jun 1, 1997·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·U Jung, K Ley
Feb 7, 1998·The Journal of Experimental Medicine·T G DiacovoU H von Andrian
May 23, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·Y NakashimaR Ross
Jul 3, 1998·The Journal of Clinical Investigation·Z M DongD D Wagner
Jan 14, 1999·The New England Journal of Medicine·R Ross
May 26, 1999·Cardiovascular Research·A C van der Wal, A E Becker
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E J KunkelK Ley

❮ Previous
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Citations

Feb 27, 2008·Annals of Biomedical Engineering·Christopher D Paschall, Michael B Lawrence
Oct 11, 2002·Free Radical Biology & Medicine·Karen Y StokesD Neil Granger
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniel CruzOhyun Kwon
Oct 9, 2002·The Journal of Experimental Medicine·Steffen MassbergMeinrad Gawaz
May 8, 2009·Nanotechnology·Ashwath JayagopalFrederick R Haselton
Sep 14, 2004·Current Opinion in Lipidology·Einar E Eriksson
Mar 26, 2011·Thrombosis and Haemostasis·R T A MegensO Soehnlein
Mar 20, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Miina K OhmanDaniel T Eitzman
Jul 16, 2011·PloS One·Izabela RozenbergFelix C Tanner
Oct 1, 2011·International Journal of Vascular Medicine·Boudewijn KlopManuel Castro Cabezas
Nov 12, 2013·Seminars in Immunopathology·S Garrido-UrbaniB A Imhof
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Filip K SwirskiRalph Weissleder
May 29, 2014·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·Marijke A de VriesManuel Castro Cabezas
Feb 25, 2009·Seminars in Immunopathology·Oliver Soehnlein, Christian Weber
Nov 8, 2005·Scandinavian Journal of Clinical and Laboratory Investigation·F MøllerL B Nielsen
May 23, 2015·Thrombosis and Haemostasis·Matthias HokeGeorg Goliasch
Mar 10, 2010·Journal of Biomedical Optics·Remco T A MegensMarc A M J van Zandvoort
Oct 21, 2011·Thrombosis and Haemostasis·M DrechslerO Soehnlein
Mar 27, 2009·European Journal of Clinical Investigation·G HuangY-H Yin
Aug 13, 2003·Journal of Thrombosis and Haemostasis : JTH·C DavisI J Sarembock
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·S LindemannM Gawaz
Oct 19, 2004·Atherosclerosis·A J H H M van OostromM Castro Cabezas
Sep 3, 2005·Atherosclerosis·J P H van WijkE J P de Koning
May 18, 2010·The American Journal of Pathology·Pierre RotziusEinar E Eriksson
May 15, 2013·Clinical Science·Kevin J Woollard
Oct 11, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Karen Y StokesD Neil Granger
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S SudheendranR J Deckelbaum
Jan 27, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Byoung-Jin ParkYong-Jae Lee
Jul 25, 2006·Biophysical Journal·Gregor KnönerHalina Rubinsztein-Dunlop
Jun 9, 2016·The Journal of Experimental Medicine·Sara McArdleKlaus Ley

❮ Previous
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