PMID: 2495534Apr 1, 1989Paper

Direct visualization of the interrelationship between intramembrane and extracellular structures

Proceedings of the National Academy of Sciences of the United States of America
J B Wade, R A Coleman

Abstract

The apical surface of the toad urinary bladder is covered by an interconnected mesh of glycocalyx, which appears to attach to the plasma membrane bilayer. To evaluate the interrelationship between these extracellular elements and intramembrane structures, a strategy was devised to produce composite replicas that allow the simultaneous visualization of intramembrane particles by freeze-fracture while the glycocalyx mesh is replicated by rotary shadowing of the extracellular surface after freeze-drying. Evaluation of these composite replicas by electron microscopy reveals that contacts occur between extracellular filamentous elements and intramembrane particles. This structural organization may be important for stabilizing intramembrane components and for anchoring extracellular elements to the membrane.

References

Oct 3, 1975·Science·W A KachadorianV A DiScala
Dec 1, 1975·The Journal of Cell Biology·M Dubois-Dalcq, T S Reese
Feb 1, 1986·The Journal of Cell Biology·K A Fisher, K C Yanagimoto
Dec 1, 1974·The Journal of Cell Biology·A Elgsaeter, D Branton
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K Hong, W L Hubbell
Jun 1, 1970·The Journal of Cell Biology·P Pinto da Silva, D Branton
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D Branton
Apr 17, 1980·Nature·S Gluck, Q Al-Awqati
Jan 1, 1984·The Journal of Membrane Biology·W Almers, C Stirling
Feb 1, 1983·Journal of Ultrastructure Research·K R MillerN L Lassignal
Sep 1, 1984·The Journal of Cell Biology·P Pinto da Silva, F W Kan
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D E Golan, W Veatch
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D E KoppelM Schindler

❮ Previous
Next ❯

Citations

Jan 1, 1989·Electron Microscopy Reviews·M V Nermut
Mar 31, 2012·American Journal of Physiology. Cell Physiology·Dennis BrownHua A J Lu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.