PMID: 8601307Mar 22, 1996Paper

Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing

Cell
S D SeiwertK Stuart

Abstract

Deletion of uridylates from the 3'-most editing site of synthetic ATPase 6 pre-mRNA can be visualized directly by coincubation of a radiolabeled substrate RNA and a synthetic gRNA in 20S fractions of T.brucie mitochondrial lysates. Substrate RNA cleavage is gRNA directed and occurs 3' to the uridylates to be deleted. U residues appear to be sequentially removed from the 3' end of the 5' cleavage product prior to religation of the two pre-mRNA halves. gRNA/mRNA chimeric molecules are also produced. Time course experiments indicate that chimeras appear after cleavage intermediates and edited product. Furthermore, a mutant gRNA promotes formation of edited product but not detectable chimeras. Our results suggest a model for kinetoplastid RNA editing in which chimeric molecules are nonproductive end products of editing and not intermediates that serve as a repository for deleted U's.

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Citations

Oct 12, 2001·International Journal for Parasitology·A Schneider
Jun 14, 2002·International Journal for Parasitology·Lisa M Oppegard, Gregory J Connell
Mar 25, 2000·International Journal for Parasitology·J E Feagin
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Feb 19, 2002·Molecular and Biochemical Parasitology·Reza SalavatiKenneth Stuart
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Jul 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Feng LiLarry Simpson
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Cruz-Reyes, B Sollner-Webb
Mar 8, 2011·The Journal of Biological Chemistry·Houtan MoshiriReza Salavati
Apr 9, 2011·The Journal of Biological Chemistry·Jason CarnesKenneth Stuart
Mar 17, 2001·The EMBO Journal·U F MüllerH U Göringer
Apr 3, 2002·The EMBO Journal·Maciej DrozdzKenneth Stuart
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May 2, 2012·Molecular and Cellular Biology·Igor Y MorozovMark X Caddick
Oct 24, 2007·Molecular and Cellular Biology·Jason CarnesKenneth Stuart

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