Direct-write fabrication of functional protein matrixes using a low-cost Q-switched laser

Analytical Chemistry
Bryan KaehrJason B Shear

Abstract

We report the use of an inexpensive, small, and "turn-key" Q-switched 532-nm Nd:YAG laser as a source for nonlinear, direct-write protein microfabrication. In this approach, microJoule pulses (pulse widths, approximately 600 ps) are focused using high numerical aperture optics to submicrometer focal spots, creating instantaneous intensities great enough to promote multiphoton excitation of a photosensitizer and subsequent intermolecular cross-linking of protein molecules. By scanning the femtoliter focal volume through reagent solution, extended protein-based structures can be fabricated with precise, three-dimensional topographies. As with earlier studies using a femtosecond titanium:sapphire laser costing more than 100K, physically robust and chemically responsive microstructures can be fashioned rapidly with feature sizes smaller than 0.5 microm, and cross-linking can be achieved using both biologically benign sensitizers (e.g., flavins) and by using the proteins themselves to sensitize cross-linking. We demonstrate in situ fabrication to corral neurite outgrowth and show the ability to functionalize avidin structures with biotinylated reagents, an approach that enables chemical sensing to be performed in specified microenvi...Continue Reading

References

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Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bryan KaehrJason B Shear
Aug 16, 2005·Analytical Chemistry·Richard AllenJason B Shear

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Citations

Apr 1, 2008·Nanomedicine·Stephanie K SeidlitsChristine E Schmidt
Feb 16, 2007·Nature Chemical Biology·Rami N Hannoush
Jul 6, 2014·Analytical Chemistry·Eric C SpiveyIlya J Finkelstein
Jun 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Bryan Kaehr, Jason B Shear
Jul 27, 2007·Angewandte Chemie·Christopher N LaFrattaRichard A Farrer
Aug 26, 2009·Lab on a Chip·Bryan Kaehr, Jason B Shear
Oct 1, 2008·Optics Express·Nathan J JennessRobert L Clark
Sep 4, 2015·Biofabrication·Alex BellVasile Nistor
Jan 28, 2012·Lab on a Chip·Eric T RitschdorffJason B Shear
Jan 1, 2020·Nanomaterials·Liangliang YangXiaodong Wang
May 18, 2019·Light, Science & Applications·Qiming ZhangMin Gu
Jun 4, 2020·Advanced Materials Technologies·Alexandra P KuoAlbert Folch
Jan 20, 2021·ACS Biomaterials Science & Engineering·Daniela Serien, Koji Sugioka
Sep 30, 2010·Analytical Chemistry·Eric T Ritschdorff, Jason B Shear
Nov 3, 2020·ACS Applied Nano Materials·Puskal Kunwar, Pranav Soman
Jan 31, 2007·Journal of the American Chemical Society·Bryan Kaehr, Jason B Shear
Sep 30, 2006·Analytical Chemistry·Ryan T Hill, Jason B Shear

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