Direct xylan conversion into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048(T)

Enzyme and Microbial Technology
Nuno Torres FariaFrederico Castelo Ferreira

Abstract

Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048(T) and Pseudozyma aphidis PYCC 5535(T) to use cellulose (Avicel(®)) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel(®) nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048(T) cultures. MEL titers reached 1.3g/l after 10 days in batch cultures with 40g/l xylan, and 2.0g/l in fed-batch cultures with xylan feeding (additional 40g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47-62U/ml (31-32U/mg) at 50°C, and still exhibiting more than 10U/ml under physiological temperature (28°C)....Continue Reading

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Citations

Jun 6, 2020·World Journal of Microbiology & Biotechnology·Chandraprasad MadihalliMukesh Doble
Sep 19, 2017·Applied Microbiology and Biotechnology·Bruno N PaulinoGlaucia M Pastore
Jun 28, 2019·Frontiers in Molecular Neuroscience·Sara TabbaiCarmen Pedraza
Apr 17, 2021·Essays in Biochemistry·Nick WierckxKerstin Schipper
Jun 17, 2021·Bioprocess and Biosystems Engineering·André Felipe da SilvaDiogo Robl

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