PMID: 2510819Aug 22, 1989Paper

Directed alteration of the D1 polypeptide of photosystem II: evidence that tyrosine-161 is the redox component, Z, connecting the oxygen-evolving complex to the primary electron donor, P680

Biochemistry
J G MetzB A Diner

Abstract

In photosystem II, electrons are sequentially extracted from water at a site containing Mn atoms and transferred through an intermediate carrier (Z) to the photooxidized reaction-center chlorophyll (P680+). Two polypeptides, D1 and D2, coordinate the primary photoreactants of the reaction center. Recently Debus et al. [Debus, R.J., Barry, B.A., Babcock, G.T., & McIntosh, L. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 427-430], have suggested that Z is a tyrosine residue located at position 161 of the D1 protein. To test this proposal, we have engineered a strain of the cyanobacterium Synechocystis PCC 6803 to produce a D1 polypeptide in which Tyr-161 has been replaced by phenylalanine. Wild-type Synechocystis PCC 6803 contains three nonidentical copies of the psbA gene which encode the D1 polypeptide. In the mutant strain, two copies were deleted by replacement with antibiotic-resistance genes, and site-directed mutations were constructed in a cloned portion of the remaining gene (psbA-3), carrying a third antibiotic-resistance gene downstream. Transformants were selected for antibiotic resistance and then screened for a photoautotrophy-minus phenotype. The mutant genotype was verified by complementation tests and by amplification...Continue Reading

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Citations

Aug 1, 1991·Photosynthesis Research·H T Witt
May 1, 1996·Photosynthesis Research·R FiegeW Lubitz
Jan 1, 1993·Photosynthesis Research·W F VermaasB Andersson
Jan 1, 1993·Photosynthesis Research·K P BaderG H Schmid
Nov 1, 1993·Photosynthesis Research·H Huzisige, B Ke
Sep 20, 2005·Photosynthesis Research·Sergey KosourovMaria L Ghirardi
Jul 25, 2007·Photosynthesis Research·Gernot Renger
Jul 20, 2007·Photosynthesis Research·Jan Kern, Gernot Renger
May 17, 2008·Photosynthesis Research·Eduardo M SprovieroVictor S Batista
Oct 6, 2010·ACS Chemical Biology·Robin S SibertBridgette A Barry
Jul 1, 2011·Journal of the American Chemical Society·James M KeoughBridgette A Barry
Dec 31, 2011·Journal of the American Chemical Society·Toshihiro TakashimaRyuhei Nakamura
Nov 25, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·James P McEvoyGary W Brudvig
Aug 9, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Arianna BarbafinaMassimo Trotta
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R A RoffeyR T Sayre
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G T Babcock
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G M MacDonaldB A Barry
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V P Shinkarev, C A Wraight
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·C BüchelC Dismukes
Nov 20, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wolfgang JungeJürgen Clausen
Oct 30, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Eberhard SchlodderBruce A Diner
Jan 1, 1993·Photochemistry and Photobiology·B A Barry
May 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A Rashid, R Popovic
Jan 1, 1998·Bioscience, Biotechnology, and Biochemistry·T EndoF Sato
May 3, 2011·Journal of Photochemistry and Photobiology. B, Biology·Harvey J M Hou, David Mauzerall

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