PMID: 9544938Apr 17, 1998Paper

Directed biosynthesis of peptaibol antibiotics in two Trichoderma strains. I. Fermentation and isolation

The Journal of Antibiotics
G LeclercB Bodo

Abstract

Peptaibols are linear alpha-aminoisobutyric acid-containing peptide antibiotics originating from soil fungi mainly of the genus Trichoderma and biosynthesized in complex mixtures of closely related analogues by a polyenzymatic pathway. Addition of amino acids such as alpha-aminoisobutyric acid (Aib), glutamic acid or arginine, to the fermentation medium of two Trichoderma strains, T. harzianum and T. longibrachiatum, has been shown to result in the simplification of the natural peptaibol mixtures, leading in each case to the almost exclusive biosynthesis of a single peptide. Surprisingly, the obtained peptides are Aib-enriched, whether the added amino acid is Aib, Glu or Arg. By adding Aib to the fermentation medium of T. harzianum, two new Aib-rich peptaibols were isolated. Moreover, adding glutamic acid to the culture medium of T. longibrachiatum, which produces both neutral and acidic 20-residue peptaibols with either glutamine or glutamic acid at position 18, increases the production of the acidic peptides. However, arginine which is a positively charged amino acid generally absent from peptaibol sequences, is not incorporated in trichorzins when added to the fermentation medium of T. harzianum.

Citations

Dec 9, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Thomas DegenkolbUdo Gräfe
Sep 26, 2008·Chemistry & Biodiversity·Thomas Degenkolb, Hans Brückner
Sep 26, 2008·Chemistry & Biodiversity·Chanikul ChutrakulJohn F Peberdy
May 18, 2013·Chemistry & Biodiversity·Anastase Kimonyo, Hans Brückner
Jun 30, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sloan AyersNicholas H Oberlies
Nov 17, 2007·Rapid Communications in Mass Spectrometry : RCM·Norbert StoppacherRainer Schuhmacher
Dec 9, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gábor BunkócziGeorge M Sheldrick
Jun 26, 2007·Chemistry & Biodiversity·Christian P KubicekIrina S Druzhinina
Jun 21, 2018·World Journal of Microbiology & Biotechnology·Ines TouatiFerid Limam
Feb 13, 2021·Bioscience, Biotechnology, and Biochemistry·Mira Syahfriena Amir RawaHiroyuki Osada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.