Directed Differentiation of Human Corneal Endothelial Cells From Human Embryonic Stem Cells by Using Cell-Conditioned Culture Media

Investigative Ophthalmology & Visual Science
Xiaoniao ChenLiqiang Wang

Abstract

A shortage of human corneal endothelial cells (HCEC) for transplant and current methods of differentiation induction require chemical compounds, which might cast further influences after differentiation induction. Therefore, we developed a simple and straightforward approach to endothelial cell differentiation from human embryonic stem cells (hESC). HESC are used to differentiate into HCEC by employing a two-stage method, which involves the application of two different types of conditioned culture medium, human corneal stromal cell-conditioned medium (HCSC-CM) and lens epithelial cell (LEC) plus HCSC-CM (LEC-CM+HCEC-CM). In brief, hESCs were treated with different conditioned media to induce directed endothelial differentiation. In the presence of conditioned culture medium, embryonic stem cells differentiate first under the control of periocular mesenchymal precursors (POMPs). Consequently, the expression of several POMP markers was observed. Following this first stage differentiation, POMPs were further directed to differentiate into corneal endothelial cell (CEC)-like cells in the presence of the second-conditioned culture medium. The differentiation of POMPs into CEC-like cells is regulated by a TGFβ-2/FOXC1 signaling pathw...Continue Reading

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Dec 29, 1998·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·J L UbelsR W Curley
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Oct 27, 2005·Investigative Ophthalmology & Visual Science·Philip J GageTord Hjalt
Dec 13, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shizuya Saika
Jan 6, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Darrell J R Evans, Drew M Noden
Aug 8, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joseph YangTeruo Okano
Sep 27, 2006·Human Molecular Genetics·Yahya TamimiMichael A Walter
Apr 24, 2009·Investigative Ophthalmology & Visual Science·Naoki OkumuraShigeru Kinoshita
Sep 3, 2009·Tissue Engineering. Part C, Methods·Ryuhei HayashiKohji Nishida
Apr 2, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazim H NarsinhJoseph C Wu
Sep 5, 2012·Indian Journal of Ophthalmology·Serge Resnikoff, Tricia U Keys
Oct 17, 2012·Cold Spring Harbor Perspectives in Biology·Whitney Heavner, Larysa Pevny
Dec 12, 2012·Current Topics in Microbiology and Immunology·Jonathan J HenryKimberly J Perry
Nov 26, 2013·Cell Transplantation·Gary S L PehJodhbir S Mehta
Jan 8, 2014·BioMed Research International·Yoshinori Oie, Kohji Nishida
Aug 12, 2014·The Lancet Global Health·Rupert R A BourneUNKNOWN Vision Loss Expert Group
May 30, 2015·Regenerative Medicine·Songtao Yuan, Guoping Fan
Jun 1, 2015·Methods in Molecular Biology·Andrew J Hertsenberg, James L Funderburgh
Jun 6, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Antionette L Williams, Brenda L Bohnsack
Aug 28, 2015·Progress in Molecular Biology and Translational Science·Peter Y Lwigale
Sep 18, 2015·Journal of Functional Biomaterials·Jesintha NavaratnamAboulghassem Shahdadfar
Apr 26, 2016·Journal of Tissue Engineering and Regenerative Medicine·Yu Qiang SohJodhbir S Mehta
Dec 22, 2016·Investigative Ophthalmology & Visual Science·Jiagang J Zhao, Natalie A Afshari
Mar 3, 2017·Investigative Ophthalmology & Visual Science·Seungwoon SeoTsutomu Kume
Feb 16, 2018·Science Translational Medicine·Dinesh JaishankarDeepak Shukla

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