Directed differentiation of mouse P19 embryonal carcinoma cells to neural cells in a serum- and retinoic acid-free culture medium

In Vitro Cellular & Developmental Biology. Animal
Isha Verma, Polani B Seshagiri

Abstract

P19 embryonal carcinoma cells (EC-cells) provide a simple and robust culture system for studying neural development. Most protocols developed so far for directing neural differentiation of P19 cells depend on the use of culture medium supplemented with retinoic acid (RA) and serum, which has an undefined composition. Hence, such protocols are not suitable for many molecular studies. In this study, we achieved neural differentiation of P19 cells in a serum- and RA-free culture medium by employing the knockout serum replacement (KSR) supplement. In the KSR-containing medium, P19 cells underwent predominant differentiation into neural lineage and by day 12 of culture, neural cells were present in 100% of P19-derived embryoid bodies (EBs). This was consistently accompanied by the increased expression of various neural lineage-associated markers during the course of differentiation. P19-derived neural cells comprised of NES+ neural progenitors (~ 46%), TUBB3+ immature neurons (~ 6%), MAP2+ mature neurons (~ 2%), and GFAP+ astrocytes (~ 50%). A heterogeneous neuronal population consisting of glutamatergic, GABAergic, serotonergic, and dopaminergic neurons was generated. Taken together, our study shows that the KSR medium is suitable ...Continue Reading

References

Feb 1, 1982·Developmental Biology·M W McBurney, B J Rogers
Aug 15, 1995·Journal of Neuroscience Research·M YaoD I Gottlieb
May 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G BainD I Gottlieb
May 25, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter W Andrews
Aug 15, 2002·Cell·Hynek WichterleThomas M Jessell
Jun 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sheng DingPeter G Schultz
Jul 6, 2004·Molecular and Cellular Neurosciences·Yiping ShenKarina F Meiri
Sep 4, 2004·Stem Cells and Development·S A PrzyborskiG M Horrocks
Apr 28, 2006·Molecular Biology of the Cell·Chen WangNaihe Jing
Jan 3, 2008·PloS One·Francesc R Garcia-Gonzalo, Juan Carlos Izpisúa Belmonte
Oct 30, 2008·Biochemical and Biophysical Research Communications·Sukchul HongSungyoul Hong
Jan 6, 2009·Neuroscience Letters·Takeshi Takarada, Yukio Yoneda
Mar 13, 2009·Acta Biochimica Et Biophysica Sinica·Bing HuangYouxin Jin
Oct 8, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mathieu SolariMonique Boily
Aug 14, 2010·Human Reproduction Update·Ewart W KuijkBernard A J Roelen
Oct 1, 2010·Neurochemical Research·Silvia ParisiTommaso Russo
Jun 16, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takeshi ItoYoshinori Murakami
Nov 22, 2011·Journal of Neuroscience Methods·Hector J MonzoMaurice A Curtis
May 30, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gia-Ming Hong, Lisa J Bain
Oct 31, 2013·BioMed Research International·Nopporn Jongkamonwiwat, Parinya Noisa
Mar 4, 2014·Journal of Neuroscience Methods·Yoshiaki NakayamaAkira Kurosaka
Jul 22, 2014·Angewandte Chemie·Gun-Hee KimInjae Shin
Jan 29, 2016·Nature Reviews. Molecular Cell Biology·Yishai AviorNissim Benvenisty
Jun 17, 2016·Anti-cancer Agents in Medicinal Chemistry·Jyotshna Kanungo

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BETA
flow cytometry
PCR
Fluorescence

Software Mentioned

ProteinSimple
GraphPad PRISM
Image
StepOne
AlphaEaseFC
GraphPad
Leica Application Suite Advanced Fluorescence ( LAS AF ) Lite
Pro Plus
BD FACSDiva
AlphaSnap

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