Directed evolution of proteins for increased stability and expression using yeast display

Archives of Biochemistry and Biophysics
Michael W Traxlmayr, Christian Obinger

Abstract

The expression of recombinant proteins incorporated into the cell wall of Saccharomyces cerevisiae (yeast surface display) is an important tool for protein engineering and library screening applications. In this review, we discuss the state-of-the-art yeast display techniques used for stability engineering of proteins including antibody fragments and immunoglobulin-like molecules. The paper discusses assets and drawbacks of stability engineering using the correlation between expression density on the yeast surface and thermal stability with respect to the quality control system in yeast. Additionally, strategies based on heat incubation of surface displayed protein libraries for selection of stabilized variants are reported including a recently developed method that allows stabilization of proteins of already high intrinsic thermal stability like IgG1-Fc.

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Citations

Dec 26, 2012·Protein Engineering, Design & Selection : PEDS·Michael W TraxlmayrChristian Obinger
May 7, 2013·Annual Review of Biomedical Engineering·Scott BantaOren Shur
Feb 13, 2016·Immunological Reviews·Elisabeth LobnerChristoph Hasenhindl
Dec 3, 2013·FEBS Letters·Achim DoernerHarald Kolmar
Oct 29, 2015·Protein Science : a Publication of the Protein Society·Marlies FischerNicholas Pj Brindle
Sep 25, 2015·Immunogenetics·Ana PinheiroPedro José Esteves
Mar 17, 2015·Scientific Reports·Emilio LamazaresJavier Sancho
Jan 6, 2015·The Journal of Pharmacy and Pharmacology·Roslyn M Bill
Feb 14, 2014·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Miriam Kaltenbach, Nobuhiko Tokuriki
Jun 26, 2014·Biotechnology Journal·Michael W TraxlmayrChristian Obinger
Sep 20, 2015·Protein Engineering, Design & Selection : PEDS·Mark C JulianPeter M Tessier
Jan 1, 2012·Computational and Structural Biotechnology Journal·Azadeh Pourmir, Tyler W Johannes
Sep 5, 2012·Archives of Biochemistry and Biophysics·Florian Rüker
Sep 24, 2015·Chembiochem : a European Journal of Chemical Biology·Brennal PierreJin Ryoun Kim
Sep 2, 2018·Biotechnology Journal·Maryam Raeeszadeh-SarmazdehEric T Boder
Jun 4, 2019·The FEBS Journal·Jiří ZahradníkBohdan Schneider
Sep 2, 2016·The Journal of Biological Chemistry·Michael W TraxlmayrK Dane Wittrup
Jan 29, 2021·Viruses·Kazuya Nagano, Yasuo Tsutsumi
Oct 16, 2020·International Journal of Molecular Sciences·Manali S SawantPeter M Tessier
Jun 26, 2021·Cell Host & Microbe·Chihiro MotozonoKei Sato

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