Directional control of WAVE2 membrane targeting by EB1 and phosphatidylinositol 3,4,5-triphosphate

Cellular Signalling
Kazuhide TakahashiKatsuo Suzuki

Abstract

Membrane targeting of WAVE2 along microtubules is mediated by a motor protein kinesin and requires Pak1, a downstream effector of Rac1. However, the mechanism by which WAVE2 targeting to the leading edge is directionally controlled remains largely unknown. Here we demonstrate that EB1, a microtubule plus-end-binding protein, constitutively associates with stathmin, a microtubule-destabilizing protein, in human breast cancer cells. Stimulation of the cells with insulin-like growth factor I (IGF-I) induced Pak1-dependent binding of the EB1-stathmin complex to microtubules that bear WAVE2 and colocalization of the complex with WAVE2 at the leading edge. Depletion of EB1 by small interfering RNA (siRNA) abrogated the IGF-I-induced WAVE2 targeting and stathmin binding to microtubules. On the other hand, chemotaxis chamber assays indicated that the IGF-I receptor (IGF-IR) was locally activated in the region facing toward IGF-I. In addition, IGF-I caused phosphatidylinositol 3-kinase (PI 3-kinase)-dependent production of phosphatidylinositol 3,4,5-triphosphate (PIP3) near activated IGF-IR and WAVE2 colocalization with it. Collectively, WAVE2-membrane targeting is directionally controlled by binding of the EB1-stathmin complex to WAVE2...Continue Reading

References

Feb 9, 1996·Cell·T J Mitchison, L P Cramer
Mar 28, 1997·The Journal of Biological Chemistry·S B HorwitzU K Schubart
Feb 7, 1998·Science·A Hall
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Sep 20, 2000·The Journal of Cell Biology·R RohatgiM W Kirschner
Dec 21, 2000·The Journal of Cell Biology·J M HaughT Meyer
Jun 8, 2001·Annual Review of Biochemistry·B VanhaesebroeckM D Waterfield
Jan 17, 2002·Current Opinion in Cell Biology·Lynne Cassimeris
Jan 9, 2003·Current Opinion in Cell Biology·Niels Galjart, Franck Perez
Jan 29, 2003·The Journal of Cell Biology·Supriya SrinivasanHenry R Bourne
Apr 18, 2003·Nature·Joe Howard, Anthony A Hyman
Oct 17, 2003·Journal of Biochemistry·Yuko Mimori-Kiyosue, Shoichiro Tsukita
Dec 3, 2003·The Journal of Biological Chemistry·Torsten WittmannClare M Waterman-Storer
Apr 27, 2004·Nature Cell Biology·Tsukasa OikawaTadaomi Takenawa
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuan GaoYi Zheng
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Peter J M Van Haastert, Peter N Devreotes
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yan LengKatherine Siminovitch
Feb 25, 2005·Molecular Biology of the Cell·Scott A MyersChang Y Chung
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Niels Galjart
Jul 11, 2006·Developmental Cell·Gideon LansbergenAnna Akhmanova
Mar 7, 2008·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Mar 4, 2009·The Journal of Cell Biology·Yulia KomarovaAnna Akhmanova

❮ Previous
Next ❯

Citations

Oct 1, 2011·International Journal of Cell Biology·Shigeru Morimura, Kazuhide Takahashi
Mar 19, 2013·Seminars in Cell & Developmental Biology·Atsushi Oda, Koji Eto
Mar 13, 2012·Plant Science : an International Journal of Experimental Plant Biology·Laura GleesonSherryl R Bisgrove
Feb 9, 2012·Journal of Oncology·Kazuhide Takahashi
Dec 28, 2010·Biochemical and Biophysical Research Communications·Shigeru MorimuraKazuhide Takahashi
Jun 18, 2015·The Journal of Biological Chemistry·Kewei Xu, Rene E Harrison

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.