Directional freezing of sperm and associated derived technologies

Animal Reproduction Science
Amir Arav, Joseph Saragusty

Abstract

Directional freezing has now completed 30 years of development since it was first introduced to cryobiology. In the field of sperm cryopreservation, directional freezing has been shown to be advantageous over slow freezing for numerous domestic and wildlife species. In particular, it was shown that freezing of large volume is possible. Furthermore, double freezing of sperm and freezing of sex-sorted sperm are possible and became the routine in the sex sorted sperm industry. In wild animals, our labs and others showed that sperm from a wide range of terrestrial and aquatic species can be successfully cryopreserved using directional freezing. Finally, we will describe for the first time the successful freeze-drying of human sperm in an aseptic method. Using a device that produces clean liquid air, we froze human sperm in small droplets and then dried them in a bench top lyophilizer that was sterilized prior to use. More than 80% of DNA integrity was found after rehydration.

References

Sep 1, 1988·Biophysical Journal·D E Pegg, M P Diaper
Jul 14, 1998·Nature Biotechnology·T Wakayama, R Yanagimachi
Jul 24, 2002·Biology of Reproduction·Levent KeskintepeBenjamin G Brackett
Sep 21, 1956·Science·H T MERYMAN
Feb 13, 2004·Biology of Reproduction·Ji-Long LiuXiangzhong Yang
Jun 30, 2005·Zygote : the Biology of Gametes and Early Embryos·Masumi HirabayashiShinichi Hochi
Oct 11, 2007·Molecular Reproduction and Development·Shinichi HochiMasumi Hirabayashi
Nov 3, 2007·Reproduction in Domestic Animals = Zuchthygiene·J SaragustyA Arav
Nov 15, 2008·Theriogenology·R HermesT B Hildebrandt
Dec 26, 2008·Animal Reproduction Science·Joseph SaragustyAmir Arav
Dec 30, 2008·Animal Reproduction Science·Joseph SaragustyRobert Hermes
Feb 14, 2009·Theriogenology·B W W Grout, G J Morris
Mar 21, 2009·Theriogenology·J SaragustyA Arav
Jul 14, 2009·Biotechnology and Bioengineering·Joseph SaragustyAmir Arav
Oct 27, 2009·Journal of Assisted Reproduction and Genetics·Hiroyuki WatanabeHiroshi Suzuki
Feb 18, 2011·The Journal of Reproduction and Development·Hiromasa HaraShinichi Hochi
Aug 19, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Y H ChoiK Hinrichs
Mar 6, 2012·Fertility and Sterility·Luca GianaroliAnna Pia Ferraretti
Mar 14, 2013·PloS One·Robert HermesThomas B Hildebrandt
Nov 20, 2014·PloS One·Takehito KanekoMiho Inoue-Murayama
Feb 3, 2015·Reproductive Biomedicine Online·Gábor VajtaFilippo Maria Ubaldi
Mar 13, 2015·Reproduction, Fertility, and Development·Joseph SaragustyRobert Hermes
Aug 8, 2015·Cryobiology·M OlacireguiL Gil
Sep 10, 2015·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·María Teresa Prieto PablosRobert Hermes
Jan 26, 2016·Theriogenology·Joseph SaragustyThomas Bernd Hildebrandt

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Citations

Jul 6, 2018·The Journal of Reproduction and Development·Luca PalazzeseJoseph Saragusty
Jan 27, 2019·Reproduction in Domestic Animals = Zuchthygiene·Laura Guadalupe GrötterLuis B Ferré
Mar 15, 2018·Journal of Assisted Reproduction and Genetics·Amir AravSergio Ledda
Dec 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kankan QinFrancisco M Fernandes
Jul 3, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Arto MerivaaraMarjo Yliperttula
Aug 18, 2021·Cryobiology·Akalabya Bissoyi, Ido Braslavsky

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