Directly energized uptake of beta-estradiol 17-(beta-D-glucuronide) in plant vacuoles is strongly stimulated by glutathione conjugates.

The Journal of Biological Chemistry
M KleinGottfried Weissenböck

Abstract

A directly energized vacuolar pump for glutathione (GS) conjugates has been described for several plant species. Since glucuronate conjugates also occur in plants, we addressed the question whether plant vacuoles take up the abiotic glucuronate conjugate estradiol 17-(beta-glucuronide) (E217G) via a GS conjugate pump, which in some cases has been reported to accept various organic anions as substrates, or via a distinct glucuronate transporter. Uptake studies into vacuoles from rye and barley were performed with E217G and metolachlor-GS (MOC-GS), a substrate of the GS conjugate ATPase, to compare glucuronate conjugate transport into vacuoles containing endogenous flavone glucuronides with those lacking specific glucuronate conjugates, respectively. Our results indicate that E217G and MOC-GS are taken up into vacuoles of both plants via a directly energized mechanism since transport was (i) strictly ATP-dependent; (ii) inhibited by vanadate but not by bafilomycin A1, azide, verapamil, nor by dissipation of the vacuolar DeltapH or DeltaPsi; (iii) E217G uptake into rye vacuoles was partially driven by other nucleotides in the following order of efficiency: ATP > GTP > UTP congruent with CTP, whereas the non-hydrolyzable ATP analog...Continue Reading

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Citations

Feb 7, 2001·Biochemical and Biophysical Research Communications·X Q RenS Akiyama
May 11, 2004·Molecular Biology of the Cell·Markus GeislerEnrico Martinoia
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Philip A. ReaEnrico Martinoia
Feb 1, 2007·Annual Review of Plant Biology·Philip A Rea
Jun 27, 2008·Phytochemical Analysis : PCA·H D SmolarzE Mendyk
Feb 4, 2012·The Arabidopsis Book·Joohyun KangEnrico Martinoia
Apr 5, 2000·Biochimica Et Biophysica Acta·F L Theodoulou

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