Disabled-2 is required for mesoderm differentiation of murine embryonic stem cells

Journal of Cellular Physiology
Chien-Ling HuangChing-Ping Tseng

Abstract

A variety of signaling networks are implicated in the control of mesoderm differentiation. Previous studies demonstrated that Disabled-2 (DAB2) is a multifunctional protein involved in growth factor signaling and embryonic development. In this study, we investigated DAB2 expression and function during in vitro mesoderm differentiation of murine embryonic stem cells (ESCs). We found that DAB2 was up-regulated when ESCs were co-cultured with OP9 stromal cells for mesoderm differentiation. DAB2 was also up-regulated when ESCs were induced for embryoid body formation. Expression of DAB2 short hairpin small interfering RNA (shDAB2) did not alter the puripotency of ESCs. However, shDAB2 disrupted ESCs cell-cell adhesion and affected embryoid body and colony formation that subsequently impeded mesoderm differentiation of ESCs. Immunofluorescent staining revealed that disorganization of beta-catenin and plakoglobin cellular distribution may account for the aberrant cell-cell adhesion in DAB2-deficient cells. Accordingly, DAB2 was identified as a plakoglobin-binding partner with the interaction mediated by the phosphotyrosine binding domain of DAB2 and the Asn-Pro-Asp-Tyr (NPDY) motif of plakoglobin. Molecular analysis and transcriptome...Continue Reading

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Citations

Jun 4, 2014·Cell Biology International·Hui-Chun ChuChing-Ping Tseng
Apr 25, 2012·Biochemical and Biophysical Research Communications·Jung Ah KimSung Soo Park
May 13, 2014·The International Journal of Biochemistry & Cell Biology·Tsai-Hsien HungKowit Yu Chong
Jun 19, 2012·Biochimica Et Biophysica Acta·Wei-Shan HungChing-Ping Tseng
Oct 22, 2016·Thrombosis Journal·Hui-Ju Tsai, Ching-Ping Tseng
Sep 13, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Hui-Ju TsaiChing-Ping Tseng
Sep 7, 2017·Journal of Thrombosis and Haemostasis : JTH·H-J TsaiC-P Tseng
Jun 16, 2015·Frontiers in Immunology·Margot Zöller
Jul 2, 2020·Cell and Tissue Research·Na ShangWood Yee Chan

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