PMID: 5200Feb 23, 1976

Disadvantages of acid precipitation of proteins during the assay of blood glucose by the enzymatic glucose oxidase-peroxidase system

Comptes rendus hebdomadaires des séances de l'Académie des sciences. Série D: Sciences naturelles
M L Van Hung

Abstract

In the enzymatic procedure for blood sugar by means of glucose oxidase, acid protein precipitation of blood by perchloric acid or trichloracetic acid liberated oxidizing substances, which enhanced the coloration density in oxidizing the reduced chromogen of the reaction mixture, independently of the hydrogen peroxide generated from glucose, and would give false high values of glycemia, if additional precautions had not been taken. These substances, increasing considerably with times and temperature of blood conservation, would be of peroxide nature, and would accumulate in red blood cells during their exposure to air.

Related Concepts

Barium
Blood Glucose
Glucose Oxidase
Hydrogen-Ion Concentration
Hydroxides
Oxidation-Reduction
Perchlorates
Sulfates, Inorganic
Sodium Trichloroacetate
Zinc

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