PMID: 8962134Dec 10, 1996Paper

Disappearance of body fat in normal rats induced by adenovirus-mediated leptin gene therapy

Proceedings of the National Academy of Sciences of the United States of America
G ChenRoger H Unger

Abstract

Sustained hyperleptinemia of 8 ng/ml was induced for 28 days in normal Wistar rats by infusing a recombinant adenovirus containing the rat leptin cDNA (AdCMV-leptin). Hyperleptinemic rats exhibited a 30-50% reduction in food intake and gained only 22 g over the experimental period versus 115-132 g in control animals that received saline infusions or a recombinant virus containing the beta-galactosidase gene (AdCMV-beta Gal). Body fat was absent in hyperleptinemic rats, whereas control rats pair-fed to the hyperleptinemic rats retained approximately 50% body fat. Further, plasma triglycerides and insulin levels were significantly lower in hyperleptinemic versus pair-fed controls, while fatty acid and glucose levels were similar in the two groups, suggestive of enhanced insulin sensitivity in the hyperleptinemic animals. Thus, despite equivalent reductions in food intake and weight gain in hyperleptinemic and pair-fed animals, identifiable fat tissue was completely ablated only in the former group, raising the possibility of a specific lipoatrophic activity for leptin.

References

Nov 1, 1983·The Journal of Clinical Investigation·E FerranniniR A DeFronzo
Jul 28, 1995·Science·M A PelleymounterF Collins
Apr 26, 1995·Biochemical and Biophysical Research Communications·T Murakami, K Shima
Jan 1, 1994·Methods in Cell Biology·T C BeckerC B Newgard
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F EngelhardtJ M Wilson
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ M Wilson
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Herz, R D Gerard
Jun 15, 1996·The Journal of Clinical Investigation·D T SteinJ D McGarry
Aug 26, 1996·FEBS Letters·M Y WangR H Unger

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Citations

Jul 13, 2000·Microscopy Research and Technique·B N Finck, R W Johnson
Sep 12, 2000·Current Hypertension Reports·W G Haynes
Sep 12, 2000·Current Hypertension Reports·L A Cassis
Oct 22, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Vairappan Balasubramaniyan, Namasivayam Nalini
Jun 11, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·S GoverdhanaM G Castro
Oct 10, 2002·Biochemical and Biophysical Research Communications·Tetsuya KakumaRoger H Unger
Apr 10, 2003·Biochemical and Biophysical Research Communications·Mary Anne Della-FeraClifton A Baile
Apr 25, 2003·Biochemical and Biophysical Research Communications·Maria Sörhede WinzellBo Ahrén
Jul 2, 2003·Physiology & Behavior·Yang-Ho ChoiClifton A Baile
Mar 21, 1998·Lancet·J Auwerx, B Staels
Oct 12, 2000·Regulatory Peptides·R H Unger
Mar 1, 2003·Regulatory Peptides·M Friedman-EinatA D Strosberg
Oct 20, 2001·Diabetes Research and Clinical Practice·C IshiiS Katayama
May 9, 2002·Molecular and Cellular Endocrinology·O GualilloF F Casanueva
Apr 3, 1998·Gene·M TaouisJ Simon
Mar 2, 2002·Domestic Animal Endocrinology·R G VernonA Sørensen
Mar 2, 2002·Domestic Animal Endocrinology·C R BarbK L Houseknecht
Dec 23, 1998·Domestic Animal Endocrinology·K L Houseknecht, C P Portocarrero
Feb 19, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·B B BoyerB M Barnes
Oct 5, 2001·Cellular Signalling·G Frühbeck, J Gómez-Ambrosi
Nov 25, 2000·Trends in Endocrinology and Metabolism : TEM·M L ReitmanS I Taylor
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·A R G Wylie
Feb 20, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·C S AsensioF Rohner-Jeanrenaud
Jul 21, 2005·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R B Ceddia
Jan 7, 1999·Clinical Endocrinology·J M Fernández-RealW Ricart-Engel
Nov 13, 2001·Diabetes, Obesity & Metabolism·M A Della-FeraC A Baile
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z W WangR H Unger
Oct 30, 2001·The Proceedings of the Nutrition Society·G Frühbeck
Dec 24, 2004·Critical Reviews in Food Science and Nutrition·Pervin BasaranMehmet Numan Tamer

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