Disappearance of chorion proteins from Bombyx mori eggs treated with HCl solution to prevent diapause

Journal of Insect Physiology
Shuichiro TsurumaruT Yaginuma

Abstract

Bombyx mori eggs enter diapause immediately after completion of mesoderm segregation. HCl treatment of approximately 24-hour-old eggs (germband formation stage) is well known to be the most effective procedure to prevent entry into diapause, although the molecular mechanism remains unclear. In this study, we examined the protein composition of diapausing and nondiapausing eggs after various HCl treatments known to prevent or break diapause and found that proteins of approximately 11 and 8 kDa disappeared immediately after HCl treatment. Partial amino acid sequences of these proteins indicated that they were members of the chorion class A protein L12 family synthesized in follicle cells. Under the hypothesis that the chorion provides a barrier to oxygen, dechorionation of diapausing eggs induces resumption of embryonic development. Hence, to test this and other hypotheses about the function of these proteins, we used 20% SDS-PAGE with Coomassie Brilliant Blue staining to trace their disappearance from embryos and eggshells after treatment with HCl under different conditions and on polyvoltine, univoltine, and bivoltine silkworm races. Even when 10-day-old diapausing eggs were treated with HCl, which did not break diapause, the 1...Continue Reading

References

Jan 1, 1984·Annual Review of Genetics·M R Goldsmith, F C Kafatos
Apr 8, 2006·Biochemical and Biophysical Research Communications·Takashi HommaToshinobu Yaginuma
Dec 8, 2009·Journal of Medical Entomology·Aiqing Li, David L Denlinger

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