Disc electrophoresis of turkey serum alkaline phosphatase isoenzymes using polyacrylamide gel. 1. Procedure.

Poultry Science
J R BeckJ H Wolford

Abstract

The isoenzymes of alkaline phosphatase in turkey serum were separated by disc electrophoresis using polyacrylamide gel as the supporting medium In this laboratory we have been unable to adapt polyacrylamide gel electrophoresis procedures for turkey serum that had been described by previous workers as successful in separating the isoenzymes of avian alkaline phosphatase. A rapid and repeatable procedure that yielded excellent resolution of alkaline phosphatase isoenzymes was devised. This procedure involved a step-by-step determination of the following: a suitable gel system, buffer system, staining procedure and sample preparation.

References

Sep 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·H R Maurer, R C Allen
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Aug 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·M L Warnock
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Oct 1, 1963·American Journal of Clinical Pathology·H F TASWELL, D M JEFFERS
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Mar 6, 1965·Lancet·K F BAMFORDT E CLEGHORN

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Citations

Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G B PrincipatoE Giovannini
Apr 1, 1988·Acta Odontologica Scandinavica·S SjögrenL Hammarström
Jun 1, 1986·Acta Odontologica Scandinavica·S SjögrenL Hammarström

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