Discharge and Role of Acetylcholine Pontomesencephalic Neurons in Cortical Activity and Sleep-Wake States Examined by Optogenetics and Juxtacellular Recording in Mice

ENeuro
Youssouf CisséBarbara E Jones

Abstract

Acetylcholine (ACh) neurons in the pontomesencephalic tegmentum (PMT) are thought to play an important role in promoting cortical activation with waking (W) and paradoxical sleep [PS; or rapid eye movement (REM)], but have yet to be proven to do so by selective stimulation and simultaneous recording of identified ACh neurons. Here, we employed optogenetics combined with juxtacellular recording and labeling of neurons in transgenic (TG) mice expressing ChR2 in choline acetyltransferase (ChAT)-synthesizing neurons. We established in vitro then in vivo in anesthetized (A) and unanesthetized (UA), head-fixed mice that photostimulation elicited a spike with short latency in neurons which could be identified by immunohistochemical staining as ACh neurons within the laterodorsal (LDT)/sublaterodorsal (SubLDT) and pedunculopontine tegmental (PPT) nuclei. Continuous light pulse stimulation during sleep evoked tonic spiking by ACh neurons that elicited a shift from irregular slow wave activity to rhythmic θ and enhanced γ activity on the cortex without behavioral arousal. With θ frequency rhythmic light pulse stimulation, ACh neurons discharged in bursts that occurred in synchrony with evoked cortical θ. During natural sleep-wake states,...Continue Reading

Citations

Jun 20, 2019·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Barbara E Jones
Oct 17, 2019·Nature Reviews. Neuroscience·Antoine R AdamantidisThomas C Gent
Dec 14, 2019·The European Journal of Neuroscience·Martin MetzgerSara J Shammah-Lagnado
Sep 26, 2019·Nature Neuroscience·Ekaterina Likhtik, Joshua P Johansen
Feb 20, 2020·Critical Reviews in Biochemistry and Molecular Biology·Xiang XuXiaofeng Jia
Jun 15, 2019·The Journal of Comparative Neurology·Debora BuenoMartin Metzger
May 28, 2020·Frontiers in Systems Neuroscience·Malinda L S TantirigamaRobert N S Sachdev
Apr 23, 2020·Current Neurovascular Research·Mehmet Bostanciklioğlu

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