Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes

Frontiers in Microbiology
Janina KrauseYvonne Mast

Abstract

Streptomyces antibiotic regulatory protein (SARP) family regulators are well-known activators of antibiotic biosynthesis in streptomycetes. The respective genes occur in various types of antibiotic gene clusters encoding, e.g., for polyketides, ribosomally and non-ribosomally synthesized peptides, or β-lactam antibiotics. We found that overexpression of the SARP-type regulator gene papR2 from Streptomyces pristinaespiralis in Streptomyces lividans leads to the activation of the silent undecylprodigiosin (Red) gene cluster. The activation happens upon the inducing function of PapR2, which takes over the regulatory role of RedD, the latter of which is the intrinsic SARP regulator of Red biosynthesis in S. lividans. Due to the broad abundance of SARP genes in different antibiotic gene clusters of various actinomycetes and the uniform activating principle of the encoded regulators, we suggest that this type of regulator is especially well suited to be used as an initiator of antibiotic biosynthesis in actinomycetes. Here, we report on a SARP-guided strategy to activate antibiotic gene clusters. As a proof of principle, we present the PapR2-driven activation of the amicetin/plicacetin gene cluster in the novel Indonesian strain isol...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 3, 2002·Molecular Microbiology·Neil BateEric Cundliffe
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·D A WiddickM J Bibb
Mar 5, 2005·Applied and Environmental Microbiology·Asuncion MartinezMarcia S Osburne
Apr 2, 2005·Current Opinion in Microbiology·Mervyn J Bibb
Nov 30, 2005·Molecular Microbiology·Jianqiang HuangStanley N Cohen
Apr 17, 2007·Journal of Molecular Biology·Akiko TanakaSueharu Horinouchi
Apr 10, 2010·Bioscience, Biotechnology, and Biochemistry·Toshihiro SuzukiHaruyasu Kinashi
Nov 26, 2010·Applied and Environmental Microbiology·Hiroyasu OnakaTamotsu Furumai
Feb 24, 2011·Microbial Biotechnology·Juan F MartínAntonio Rodríguez-García
Jul 12, 2012·BioTechniques·Xiaojing ZhangCliff S Han
Nov 13, 2012·Applied Microbiology and Biotechnology·Kozo Ochi, Takeshi Hosaka
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Gang LiuHuarong Tan
Aug 3, 2013·Journal of Industrial Microbiology & Biotechnology·Hua ZhuGilles P van Wezel
Dec 21, 2013·Microbial Cell Factories·Héctor RodríguezRamón I Santamaría
Aug 16, 2014·Applied Microbiology and Biotechnology·Yohanes Novi KurniawanTakuya Nihira
Jul 19, 2015·Applied and Environmental Microbiology·Yvonne MastEva Schinko
Nov 27, 2015·Journal of Microbiology and Biotechnology·SangJoon Mo, Yeo Joon Yoon
Feb 9, 2016·Journal of Natural Products·David J Newman, Gordon M Cragg
Jun 18, 2016·Chembiochem : a European Journal of Chemical Biology·Danyao DuYasuo Ohnishi

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Citations

Aug 29, 2020·Natural Product Reports·Jeffrey A van SantenRoger G Linington
Mar 9, 2021·Frontiers in Bioengineering and Biotechnology·Zhenyu LiuYunzi Luo
Mar 25, 2021·Cell Chemical Biology·Katharina W WexHeike Brötz-Oesterhelt
Apr 14, 2021·Annual Review of Biochemistry·Brett C CovingtonMohammad R Seyedsayamdost
Jul 25, 2021·International Journal of Molecular Sciences·Erik MingyarEvi Stegmann

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Datasets Mentioned

BETA
ACEY00000000
QXMM00000000
PRJNA489221

Methods Mentioned

BETA
reverse transcription PCR
PCR
fluorescence imaging

Software Mentioned

antiSMASH
PatScan
ClusterBlast
OSMAC
BLASTP
ChemStation

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