Abstract
This study investigated specific gene expression profiles in patients with donor-specific cytotoxic-hyporesponsiveness, reflected by cytotoxic T-lymphocyte precursor frequency (CTLpf). The effect of calcineurin inhibitor (CNI) withdrawal was studied on markers for cytotoxicity (perforin, granzyme B), apoptosis (Fas,FasL), Th1 and Th2 cytokines (IL-2, IL-10), Th1 and Th2 transcription factors (T-bet, GATA 3), Th17 transcription factor and cytokine (RORγt, IL-17), and for immune regulation/activation (CD25, FOXP3). Peripheral blood samples from renal allograft recipients (n = 18) more than two yr after transplantation with stable renal function were analyzed before and four months after CNI withdrawal. Additionally, systolic and diastolic blood pressure, cholesterol, serum creatinine and proteinuria were evaluated, and no significant differences were measured before and after CNI withdrawal. However, CNIs' discontinuation influenced peripheral gene expression profiles. After CNI withdrawal, the mRNA expression of Granzyme B, Perforin, Fas, FasL, T-bet, GATA3 and CD25 were significantly lower than during CNI treatment. After CNI discontinuation, donor-specific CTLpf decreased, while FOXP3 expression discriminated between detectabl...Continue Reading
References
Jun 25, 1992·Nature·N A Clipstone, G R Crabtree
Dec 1, 1988·International Journal of Bio-medical Computing·L W StrijboschW A Buurman
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M KrönkeW C Greene
Jun 5, 1998·The Journal of Biological Chemistry·C B KleeX Wang
Sep 27, 2000·Journal of the American Society of Nephrology : JASN·Bertram L KasiskeJennie Z Ma
Oct 3, 2000·Journal of Molecular Endocrinology·S A Bustin
Oct 4, 2000·Physiological Reviews·F Rusnak, P Mertz
Sep 26, 2001·Transplantation·B J van der MastW Weimar
Mar 22, 2002·Nature Reviews. Immunology·John J O'SheaPeter Lipsky
Apr 19, 2002·Journal of the American Society of Nephrology : JASN·Peter J H Smak GregoorTeun van Gelder
Feb 4, 2003·Nature Reviews. Immunology·Robert I LechlerLaurence A Turka
Sep 3, 2004·Nature Reviews. Immunology·Thomas R Malek, Allison L Bayer
Dec 14, 2004·Transplantation·Martin P GallagherUNKNOWN Australian Multicenter Trial of Cyclosporine Withdrawal Study Group and the ANZ Dialysis and Transplantation Registry
Feb 26, 2005·Journal of Virology·Ornella FranzeseAntonio Bertoletti
Apr 1, 2005·The New England Journal of Medicine·Jacques Dantal, Jean-Paul Soulillou
Apr 26, 2005·Transplantation Proceedings·J van de WeteringW Weimar
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Bertram L KasiskeDavid Rush
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hans J P M KoenenIrma Joosten
Jul 9, 2005·Transplantation·Carla C BaanWillem Weimar
Nov 30, 2005·Transplantation·Barbara J van der MastWillem Weimar
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marian A FernandezCheryl A Jones
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kieng B VangMichael A Farrar
Citations
Aug 28, 2012·Cellular & Molecular Immunology·Xin ZhangQuan-Xing Wang
May 23, 2012·PloS One·Toshiaki NakanoChao-Long Chen
Mar 5, 2015·Kidney International·James A Hutchinson, Edward K Geissler
Feb 28, 2013·Pediatric Transplantation·Michael M KaabakAlan K Zokoev
Apr 15, 2011·Immunological Reviews·Maria-Grazia RoncaroloRosa Bacchetta
Apr 5, 2012·Clinical and Experimental Immunology·A HoerningO Witzke
Dec 3, 2014·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Anna Gronert ÁlvarezHeiner Wedemeyer
Dec 24, 2014·International Immunopharmacology·Daniela FanigliuloFranco Laghi-Pasini
Aug 27, 2016·Tissue & Cell·Xiao-Feng SongZhen-Xing Zhang
Dec 3, 2015·PloS One·Alexis MathianMakoto Miyara
Nov 15, 2017·Hypertension·Valorie L ChiassonBrett M Mitchell
Oct 24, 2015·Drugs·Kassem SafaDavid Wojciechowski