Discontinuity and limited linkage in the homologous recombination system of a hyperthermophilic archaeon.

Journal of Bacteriology
D W Grogan, Jananie Rockwood

Abstract

Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to the recipient chromosome along with the selected marker. In 30% of the recombinants, donor markers were transferred as multiple blocks. In at least 40% of the recombinants, donor markers separated by 5 or 6 bp segregated from each other, whereas similar markers separated by 2 bp did not segregate. Among intermarker intervals, the frequency of recombination tract endpoints varied 40-fold, but in contrast to other recombination systems, it did not correlate with the length of the interval. The average length of donor tracts (161 bp) and the frequent generation of multiple tracts seemed generally consistent with the genetic properties observed previously in S. acidocaldarius conjugation. The efficiency with which short intervals of diverged pyrE sequence were incorporated into the genome raises questions about the threat of ectopic recombination in Sulfolobus spp. mediated by this apparently efficient yet permissive s...Continue Reading

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Citations

Jul 26, 2011·Archaea : an International Microbiological Journal·Cynthia J SakofskyDennis W Grogan
Jan 7, 2014·Genome Biology and Evolution·David J KrauseRachel J Whitaker
Jan 30, 2013·Biochemical Society Transactions·Changyi ZhangRachel J Whitaker
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Adit NaorUri Gophna
Oct 17, 2017·Applied and Environmental Microbiology·Changyi Zhang, Rachel J Whitaker

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