PMID: 6168768Jul 1, 1981Paper

Discontinuous synthesis of both strands at the growing fork during polyoma DNA replication in vitro

Journal of Virology
M Närkhammar-MeuthG Magnusson

Abstract

In discontinuous polyoma DNA replication, the synthesis of Okazaki fragments is primed by RNA. During viral DNA synthesis in nuclei isolated from infected cells, 40% of the nascent short DNA fragments had the polarity of the leading strand which, in theory, could have been synthesized by a continuous mechanism. To rule out that the leading strand fragments were generated by degradation of nascent DNA, they were further characterized. DNA fragments from a segment of the genome which replication forks pass in only one direction were strand separated. The sizes of the fragments from both strands were similar, suggesting that one strand was not specifically degraded. Most important, however, the majority of the Okazaki fragments of both strands were linked to RNA at their 5' ends. For identification, the RNA was labeled at the 5' ends by [beta-32P]GTP, internally by [3H]CTP, [3H]GTP, and [3H]UTP, or at the 3' ends by 32P transfer from adjacent [32P]dTMP residues. All three kinds of labeling indicated that an equal proportion of DNA fragments from the two strands was linked to RNA primers.

References

Oct 25, 1979·Nature·J Kowalski, D T Denhardt
Nov 1, 1979·Journal of Virology·G Magnusson, M G Nilsson
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B K TyeB Weiss
Jan 1, 1979·Progress in Nucleic Acid Research and Molecular Biology·T Lindahl
Apr 25, 1979·Journal of Molecular Biology·B Y TsengM Goulian
Dec 5, 1975·Journal of Molecular Biology·B Y Tseng, M Goulian
Dec 1, 1973·The Journal of General Virology·L V CrawfordA Wilde
Aug 30, 1972·Nature: New Biology·G C Fareed, N P Salzman
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G MagnussonP Reichard
Dec 30, 1972·Journal of Molecular Biology·G MagnussonP Reichard
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P ReichardG Söderman
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·F NuzzoA Falaschi
Feb 14, 1968·Journal of Molecular Biology·J G Wetmur, N Davidson
Jul 1, 1981·Journal of Virology·M Närkhammar-MeuthD T Denhardt

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