Abstract
In discontinuous polyoma DNA replication, the synthesis of Okazaki fragments is primed by RNA. During viral DNA synthesis in nuclei isolated from infected cells, 40% of the nascent short DNA fragments had the polarity of the leading strand which, in theory, could have been synthesized by a continuous mechanism. To rule out that the leading strand fragments were generated by degradation of nascent DNA, they were further characterized. DNA fragments from a segment of the genome which replication forks pass in only one direction were strand separated. The sizes of the fragments from both strands were similar, suggesting that one strand was not specifically degraded. Most important, however, the majority of the Okazaki fragments of both strands were linked to RNA at their 5' ends. For identification, the RNA was labeled at the 5' ends by [beta-32P]GTP, internally by [3H]CTP, [3H]GTP, and [3H]UTP, or at the 3' ends by 32P transfer from adjacent [32P]dTMP residues. All three kinds of labeling indicated that an equal proportion of DNA fragments from the two strands was linked to RNA primers.
References
Oct 25, 1979·Nature·J Kowalski, D T Denhardt
Feb 1, 1975·Journal of Virology·B Otto, P Reichard
Jan 1, 1975·Journal of Virology·R KamenE Ziff
Jan 1, 1977·Nucleic Acids Research·P J Flory
Nov 5, 1977·Journal of Molecular Biology·G KaufmannM L DePamphilis
Dec 1, 1977·Cell·T HunterL Bacheler
Dec 1, 1977·Cell·D Perlman, J A Huberman
Mar 1, 1978·Cell·K BrynolfP Reichard
Jul 1, 1978·Nucleic Acids Research·G KaufmannM L DePamphilis
Apr 15, 1979·Journal of Molecular Biology·R Eliasson, P Reichard
Nov 1, 1979·Journal of Virology·G Magnusson, M G Nilsson
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B K TyeB Weiss
Nov 1, 1977·Journal of Molecular Biology·C Miyamoto, D T Denhardt
Jan 1, 1979·Progress in Nucleic Acid Research and Molecular Biology·T Lindahl
Apr 25, 1979·Journal of Molecular Biology·B Y TsengM Goulian
Nov 22, 1979·Biochimica Et Biophysica Acta·E Wist
Dec 5, 1975·Journal of Molecular Biology·B Y Tseng, M Goulian
Dec 1, 1973·The Journal of General Virology·L V CrawfordA Wilde
Aug 30, 1972·Nature: New Biology·G C Fareed, N P Salzman
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G MagnussonP Reichard
Dec 30, 1972·Journal of Molecular Biology·E L WinnackerP Reichard
Dec 30, 1972·Journal of Molecular Biology·G MagnussonP Reichard
Jan 1, 1974·Journal of Virology·T Hunter, B Francke
Jun 25, 1974·Journal of Molecular Biology·J M CoffinC Weissmann
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P ReichardG Söderman
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·F NuzzoA Falaschi
Feb 14, 1968·Journal of Molecular Biology·J G Wetmur, N Davidson
Jul 1, 1981·Journal of Virology·M Närkhammar-MeuthD T Denhardt
Citations
Sep 27, 1982·Biochimica Et Biophysica Acta·J Kowalski, D T Denhardt
Jul 1, 1981·Journal of Virology·M Närkhammar-MeuthD T Denhardt
Apr 1, 1982·Cell·R T Hay, M L DePamphilis
Apr 1, 1986·Experimental Cell Research·M M Hurt, R E Moses
May 15, 1984·Journal of Molecular Biology·R T HayM L DePamphilis
May 25, 1982·Biochemistry·Y TsubotaJ A Huberman
Apr 15, 1983·Journal of Molecular Biology·D T Denhardt, C Miyamoto