Discordance between in silico & in vitro analyses of ACE inhibitory & antioxidative peptides from mixed milk tryptic whey protein hydrolysate

Journal of Food Science and Technology
Alok ChatterjeePrerna Saini

Abstract

ACE inhibitory and antioxidative peptides identified by LCMS/MS, from mixed milk (Bubalus bubalis and Bos taurus) tryptic whey protein hydrolysate, were compared with the in silico predictions. α la and ß lg sequences, both from Bubalus bubalis and Bos taurus, were used for in silico study. SWISS-PROT and BIOPEP protein libraries were accessed for prediction of peptide generation. Study observed gaps in the prediction versus actual results, which remain unaddressed in the literature. Many peptides obtained in vitro, were not reflected in in silico predictions. Differences in identified peptides in separate libraries were observed too. In in silico prediction, peptides with known biological activities were also not reflected. Predictions, towards generation of bioactive peptides, based upon in silico release of proteins and amino acid sequences from different sources and thereupon validation in relation to actual results has often been reported in research literature. Given that computer aided simulation for prediction purposes is an effective research direction, regular updating of protein libraries and an effectual integration, for more precise results, is critical. The gaps addressed between these two techniques of research, ...Continue Reading

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Citations

Sep 5, 2015·International Journal of Molecular Sciences·Piotr MinkiewiczMonika Hrynkiewicz
Jan 21, 2016·Journal of Food Science and Technology·Alok ChatterjeeYogesh Khetra
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Meisam BaratiAmin Mousavi Khaneghah
Sep 1, 2020·Journal of Agricultural and Food Chemistry·Philipp M MeissnerKarin Schwarz
Dec 19, 2020·Current Research in Food Science·Nicholas A PearmanGordon A Morris

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