Discordance between mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing and IS6110 restriction fragment length polymorphism genotyping for analysis of Mycobacterium tuberculosis Beijing strains in a setting of high incidence of tuberculosis.

Journal of Clinical Microbiology
M HanekomR M Warren

Abstract

IS6110 restriction fragment length polymorphism (RFLP) genotyping is the most widely used genotyping method to study the epidemiology of Mycobacterium tuberculosis. However, due to the complexity of the IS6110 RFLP genotyping technique, and the interpretation of RFLP data, mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping has been proposed as the new genotyping standard. This study aimed to determine the discriminatory power of different MIRU-VNTR locus combinations relative to IS6110 RFLP genotyping, using a collection of Beijing genotype M. tuberculosis strains with a well-established phylogenetic history. Clustering, diversity index, clustering concordance, concordance among unique genotypes, and divergent and convergent evolution were calculated for seven combinations of 27 different MIRU-VNTR loci and compared to IS6110 RFLP results. Our results confirmed previous findings that MIRU-VNTR genotyping can be used to estimate the extent of recent or ongoing transmission. However, molecular epidemiological linking of cases varied significantly depending on the genotyping method used. We conclude that IS6110 RFLP and MIRU-VNTR loci evolve independently and at different rates, which l...Continue Reading

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Citations

Aug 6, 2010·Journal of Clinical Microbiology·Sahal A M Al-HajojAdri G M van der Zanden
Dec 19, 2009·Journal of Clinical Microbiology·Isdore Chola ShamputaClifton E Barry
Oct 15, 2011·Journal of Clinical Microbiology·Andreas RoetzerStefan Niemann
Jun 10, 2010·PloS One·Solomon GhebremichaelGunilla Källenius
Mar 4, 2011·Future Microbiology·Midori Kato-MaedaLaura Flores
Aug 26, 2014·BMC Infectious Diseases·Ulziijargal GurjavVitali Sintchenko
Jul 14, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Edgar AbadiaChristophe Sola
Aug 14, 2010·Journal of Medical Microbiology·Alexander IndraFranz Allerberger
Jan 15, 2015·Tropical Medicine & International Health : TM & IH·Gabriel ChamieEdwin D Charlebois
Dec 23, 2014·Emerging Infectious Diseases·Cynthia B E CheeYee-Tang Wang
Nov 10, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Anita C Schürch, Dick van Soolingen
Jan 22, 2011·Molecular Diagnosis & Therapy·Sathish SankarGopalan Sridharan
Dec 5, 2018·BMC Infectious Diseases·Kent J KosterJames T Douglas
Oct 20, 2018·Microbiology Spectrum·Darío García De Viedma, Laura Pérez-Lago

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