Discordant influence of amphotericin B on epirubicin cytotoxicity in primary hepatic malignant cells collected by a new primary culture technique

Journal of Gastroenterology and Hepatology
Zu-Yau LinJung-Fa Tsai

Abstract

The purpose of this prospective study was to investigate whether amphotericin B (AmB) had any potential role in the systemic chemotherapy of primary hepatic malignancy using cancer cells collected by the authors' method of primary culture. The specimens obtained by ultrasound-guided fine-needle aspiration biopsy (22 G) from 15 patients with hepatocellular carcinoma (HCC) and one with cholangiocarcinoma were plated into culture flask without disaggregation by trypsin-ethylenediamine tetra-acetic acid solution. Six patients with HCC and one patient with cholangiocarcinoma (7/16, 44%) had successful culture and the cancer cells at the 4th passage were continuously exposed to therapeutic ranges of epirubicin (0, 0.5, 1.0, 1.5, 2.0 microg/mL) with or without the combination of 2.5 microg/mL AmB for 24 h. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was applied to evaluate the effects of the drugs. A human HCC cell line (HA 22T/VGH) was studied for comparison. Addition of AmB showed no influence on epirubicin cytotoxicity in two patients (one partial resistant HCC and one epirubicin-sensitive cholangiocarcinoma; 25%), augmentation of the epirubicin cytotoxicity in two patients (one total resistant HCC, par...Continue Reading

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Citations

Apr 26, 2015·The Kaohsiung Journal of Medical Sciences·Chun-Chieh WuWan-Long Chuang
Jun 12, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zu-Yau Lin, Wan-Long Chuang
Mar 6, 2009·European Journal of Clinical Investigation·Z Y LinY H Chuang
Aug 5, 2014·Journal of Gastroenterology and Hepatology·Zu-Yau LinWan-Long Chuang

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