Discordant Regulation of microRNA Between Multiple Experimental Models and Human Pulmonary Hypertension

Chest
Kenny SchlosserDuncan J Stewart

Abstract

The dysregulation of microRNA (miRNA) is known to contribute to the pathobiology of pulmonary arterial hypertension (PAH). However, the relationships between changes in tissue and circulating miRNA levels associated with different animal models and human pulmonary hypertension (PH) have not been defined. A set of miRNAs that have been causally implicated in PH, including miR-17, -21, -130b, -145, -204, -424, and -503, were measured by reverse transcription-quantitative polymerase chain reaction in the plasma, lung, and right ventricle of three of the most common rodent models of PH: the rat monocrotaline and SU5416 plus chronic hypoxia (SuHx) models and the mouse chronic hypoxia model. Plasma miRNA levels were also evaluated in a cohort of patients with PAH and healthy subjects. Several miRNA showed PH model-dependent perturbations in plasma and tissue levels; however, none of these were conserved across all three experimental models. Principle component analysis of miR expression changes in plasma revealed distinct clustering between rodent models, and SuHx-triggered PH showed the greatest similarity to human PAH. Changes in the plasma levels of several miRNA also correlated with changes in tissue expression. In particular, mi...Continue Reading

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Citations

Nov 17, 2015·Current Opinion in Oncology·Audrey CourboulinSébastien Bonnet
Dec 3, 2015·Journal of Cellular Physiology·Gaetano Santulli
May 8, 2016·Sleep & Breathing = Schlaf & Atmung·Vahid Mohsenin
May 24, 2016·The Journal of Clinical Investigation·Alexander M K RothmanAllan Lawrie
Sep 8, 2016·Epigenomics·Alexander Mk RothmanAllan Lawrie
Jun 16, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Rebecca Johnson KamenyJeffrey R Fineman
Aug 6, 2016·The European Respiratory Journal·Elisabetta GamenSoni Savai Pullamsetti
Aug 29, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shih-Hung TsaiShing-Jong Lin
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Dec 10, 2020·JACC. Basic to Translational Science·Cátia A Santos-FerreiraHenrique M Girão

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