PMID: 6163528May 1, 1981Paper

Discordant synthesis and secretion of human chorionic gonadotropin and subunits by cervical carcinoma cells

Cancer Research
L A ColeC V Rao

Abstract

Epidermoid carcinoma of the cervix cell lines DoT and CaSki were examined for the production of human chorionic gonadotropin (HCG) and its subunits (HCG-alpha and HCG-beta). Additionally, the effect of butyrate and other regulatory agents on synthesis and secretion was investigated. Media and cell lysates were analyzed by radioimmunoassay for HCG and HCG-beta and by radioreceptor assay for intact HCG. Although DoT and CaSki cells secreted more HCG-beta than HCG, the cell lysates contained only one-half as much HCG-beta as HCG. This raises the possibility that HCG-alpha synthesis may be limiting to the formation of intact HCG in DoT and CaSki cell lines. Our results also indicate discordance in HCG and HCG-beta production in response to butyrate. Whereas the production of HCG-beta was increased by 5 mM sodium butyrate, the synthesis and secretion of intact hCG were decreased. These results support the concept of independent regulation of alpha- and beta-subunit synthesis and secretion.

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