Discovering and verifying DNA polymorphisms in a mung bean [V. radiata (L.) R. Wilczek] collection by EcoTILLING and sequencing.

BMC Research Notes
Noelle A BarkleyTracie M Jenkins

Abstract

Vigna radiata, which is classified in the family Fabaceae, is an important economic crop and a dietary staple in many developing countries. The species radiata can be further subdivided into varieties of which the variety sublobata is currently acknowledged as the putative progenitor of radiata. EcoTILLING was employed to identify single nucleotide polymorphisms (SNPs) and small insertions/deletions (INDELS) in a collection of Vigna radiata accessions. A total of 157 DNA polymorphisms in the collection were produced from ten primer sets when using V. radiata var. sublobata as the reference. The majority of polymorphisms detected were found in putative introns. The banding patterns varied from simple to complex as the number of DNA polymorphisms between two pooled samples increased. Numerous SNPs and INDELS ranging from 4-24 and 1-6, respectively, were detected in all fragments when pooling V. radiata var. sublobata with V. radiata var. radiata. On the other hand, when accessions of V. radiata var. radiata were mixed together and digested with CEL I relatively few SNPs and no INDELS were detected. EcoTILLING was utilized to identify polymorphisms in a collection of mung bean, which previously showed limited molecular genetic div...Continue Reading

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Citations

May 21, 2013·Plant Physiology and Biochemistry : PPB·Wilma SabettaCinzia Montemurro
Nov 16, 2010·BMC Genomics·Vicente P IbizaFernando Nuez
May 14, 2011·Molecular Ecology Resources·M R GarvinA J Gharrett
May 24, 2017·Scientific Reports·Emmanouil MetzakopianAllan Bradley
Aug 1, 2009·Functional Plant Biology : FPB·Csaba PapdiLászló Szabados

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Methods Mentioned

BETA
transgenic
PCR
genotyping

Software Mentioned

EcoTILLING
TILLING
BLAST

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