Discovering new natural products directly from crude extracts by HPLC-SPE-NMR: chinane diterpenes in Harpagophytum procumbens

Journal of Natural Products
Cailean ClarksonJerzy W Jaroszewski

Abstract

HPLC-SPE-NMR experiments with crude extracts of Harpagophytum procumbens allowed the rapid identification of novel, unstable chinane-type tricyclic diterpenes (1 and 2), along with numerous other constituents. Dramatic sensitivity gains achieved with this novel hyphenated technique establish a new paradigm in natural products research: rapid and rigorous structure determination of multiple extract components, including minor components, without preparative-scale isolation.

Citations

Nov 23, 2007·Natural Product Reports·James R Hanson
Sep 4, 2012·Journal of Ethnopharmacology·Nontobeko MncwangiNigel Gericke
Jan 1, 2012·Metabolites·Daniel A DiasUte Roessner
May 24, 2008·Phytochemical Analysis : PCA·Daniel Dias, Sylvia Urban
Jan 29, 2009·Journal of Separation Science·Daniel Anthony Dias, Sylvia Urban
Sep 17, 2009·Magnetic Resonance in Chemistry : MRC·Huiru TangYulan Wang
Dec 15, 2015·Rapid Communications in Mass Spectrometry : RCM·Angelo da Cunha PintoNorberto Peporine Lopes
Aug 13, 2013·Analytical Chemistry·Velitchka V MihalevaJacques Vervoort
Feb 9, 2008·Current Opinion in Chemical Biology·Lie-Fen Shyur, Ning-Sun Yang
Sep 8, 2007·Journal of Natural Products·Kennett SprogøeJerzy W Jaroszewski

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