Discovery and characterization of tRNAIle lysidine synthetase (TilS)

FEBS Letters
Tsutomu Suzuki, Kenjyo Miyauchi

Abstract

In the bacterial decoding system, the AUA codon is deciphered as isoleucine by tRNA(Ile) bearing lysidine (L, 2-lysyl-cytidine) at the wobble position. Lysidine is an essential modification that determines both the codon and amino acid specificities of tRNA(Ile). We identified an enzyme named tRNA(Ile) lysidine synthetase (TilS) that catalyzes lysidine formation by using lysine and ATP as substrates. Biochemical studies revealed a molecular mechanism of lysidine formation that consists of two consecutive reactions involving the adenylated tRNA intermediate. In addition, we deciphered how Escherichia coli TilS specifically discriminates between tRNA(Ile) and the structurally similar tRNA(Met), which bears the same anticodon loop. Recent structural studies unveiled tRNA recognition by TilS, and a molecular basis of lysidine formation at atomic resolution.

References

Oct 24, 1997·Science·R L TatusovD J Lipman
May 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kotaro NakanishiOsamu Nureki
Jul 28, 2006·Nature·Tomoyuki NumataOsamu Nureki
Aug 4, 2007·Methods in Enzymology·Tsutomu SuzukiYuriko Sakaguchi
Mar 27, 2009·The EMBO Journal·Sarin ChimnaronkIsao Tanaka

❮ Previous
Next ❯

Citations

Dec 28, 2011·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Gregory P FournierJ Peter Gogarten
Feb 9, 2010·Nature Chemical Biology·Yoshiho IkeuchiTsutomu Suzuki
Mar 20, 2010·Nature Chemical Biology·Tamara L Hendrickson
Oct 18, 2011·Nature Structural & Molecular Biology·Takuo OsawaTomoyuki Numata
Jan 9, 2013·Nucleic Acids Research·Takaaki TaniguchiTsutomu Suzuki
Jan 30, 2015·RNA Biology·Tsutomu Suzuki, Tomoyuki Numata
Jun 28, 2015·Genome Biology and Evolution·Walker Pett, Dennis V Lavrov
May 20, 2015·Genome Génome / Conseil National De Recherches Canada·Ahmad Abu Turab NaqviMd Imtaiyaz Hassan
May 14, 2014·Frontiers in Genetics·Takashi AbeToshimichi Ikemura
Jun 7, 2014·Frontiers in Genetics·Hiroyuki Hori
Sep 12, 2015·Pakistan Journal of Biological Sciences : PJBS·Chutipong AkkasaengAran Pathanothai
Jan 23, 2016·PloS One·Sudha RavishankarVasan K Sambandamurthy
May 14, 2014·Journal of Biomolecular Screening·Adam B ShapiroJason Thresher
Oct 4, 2018·Critical Reviews in Biochemistry and Molecular Biology·Matthias FellnerJian Hu
Sep 5, 2015·Molecular Microbiology·Patrick C ThiavilleValérie de Crécy-Lagard
Jul 10, 2019·IUBMB Life·Emil M Nilsson, Rebecca W Alexander
Mar 13, 2020·Genome Biology and Evolution·Bastian Greshake TzovarasIngo Ebersberger
Mar 24, 2020·Chembiochem : a European Journal of Chemical Biology·David G SchwarkJohn D Fisk
Jul 8, 2020·International Journal of Molecular Sciences·Julia MathlinTeresa Colombo
Dec 9, 2016·BMC Genomics·David H Ardell, Ya-Ming Hou
Oct 15, 2019·Frontiers in Genetics·Dixit SharmaShailender Kumar Verma
Aug 1, 2015·Angewandte Chemie·Micah T Nelp, Vahe Bandarian
Aug 29, 2018·Nature Chemical Biology·Takaaki TaniguchiTsutomu Suzuki
Dec 19, 2019·Frontiers in Microbiology·Mohammed ImamEdouard E Galyov
Oct 22, 2020·Viruses·Garry A DuncanJames L Van Van Etten
Mar 19, 2020·Mitochondrion·Jessica M Warren, Daniel B Sloan
May 1, 2014·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Sep 5, 2021·Methods : a Companion to Methods in Enzymology·Virginie MarchandYuri Motorin
Apr 8, 2011·Current Opinion in Microbiology·Gabriela Phillips, Valérie de Crécy-Lagard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.