Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside

Journal of Biological Engineering
Ozkan Fidan, Jixun Zhan

Abstract

Endophytic microorganisms are a rich source of bioactive natural products. They are considered as promising biofertilizers and biocontrol agents due to their growth-promoting interactions with the host plants and their bioactive secondary metabolites that can help manage plant pathogens. Identification of new endophytes may lead to the discovery of novel molecules or provide new strains for production of valuable compounds. In this study, we isolated an endophytic bacterium from the leaves of Taxus chinensis, which was identified as Pseudomonas sp. 102515 based on the 16S rRNA gene sequence and physiological characteristics. Analysis of its secondary metabolites revealed that this endophytic strain produces a major product zeaxanthin diglucoside, a promising antioxidant natural product that belongs to the family of carotenoids. A carotenoid (Pscrt) biosynthetic gene cluster was amplified from this strain, and the functions of PsCrtI and PsCrtY in the biosynthesis of zeaxanthin diglucoside were characterized in Escherichia coli BL21(DE3). The entire Pscrt biosynthetic gene cluster was successfully reconstituted in E. coli BL21(DE3) and Pseudomonas putida KT2440. The production of zeaxanthin diglucoside in Pseudomonas sp. 102515 ...Continue Reading

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Citations

Jul 4, 2020·Microbiology Resource Announcements·Samira ZarvandiHassan Rokni-Zadeh
Jun 5, 2020·Current Opinion in Biotechnology·Anita Loeschcke, Stephan Thies
Apr 20, 2021·Biotechnology and Applied Biochemistry·Viviana Teresa OrlandiFabrizio Bolognese
May 29, 2021·Applied Microbiology and Biotechnology·Yachao ChenChun Li

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Datasets Mentioned

BETA
KT2440
M87280
CP001875
LC317091
LC317092
UTC00282046
MK610450
MK613929

Methods Mentioned

BETA
PCR
scanning electron microscopy

Software Mentioned

BLASTn
BLAST
AntiSMASH

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