Discovery and validation of new protein biomarkers for urothelial cancer: a prospective analysis

The Lancet Oncology
Dan TheodorescuHenry F Frierson

Abstract

Non-invasive methods for diagnosis of urothelial carcinoma have reduced specificity in patients with non-malignant genitourinary disease or other disorders. We aimed to use mass spectrometry and bioinformatics to define and validate a cancer-specific proteomic pattern. We used capillary-electrophoresis-coupled mass spectrometry to obtain polypeptide patterns from urine samples of 46 patients with urothelial carcinoma and 33 healthy volunteers. From signatures of polypeptide mass, we established a model for predicting the presence of cancer. The model was refined further by use of 366 urine samples obtained from other healthy volunteers and patients with malignant and non-malignant genitourinary disease. We estimated the proportion of correct classifications from the refined model by applying it to a masked group containing 31 patients with urothelial carcinoma, 11 healthy individuals, and 138 patients with non-malignant genitourinary disease. We also sequenced several diagnostic polypeptides for urothelial carcinoma. We identified a diagnostic urothelial-carcinoma pattern of 22 polypeptide masses. On masked assessment, prediction models based on these polypeptides correctly classified all samples of urothelial carcinoma (sensit...Continue Reading

References

Sep 11, 2001·Acta Haematologica·I Gouin-ThibaultM M Samama
May 29, 2003·The Journal of Urology·Afina S GlasKarl H Kurth
Aug 21, 2003·Artificial Intelligence in Medicine·Frank DieterleGünter Gauglitz
Jul 30, 2004·Clinical Science·Harald MischakDanilo Fliser
Jun 21, 2005·Electrophoresis·Danilo FliserHarald Mischak

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Citations

Sep 13, 2013·Der Urologe. Ausg. A·J NeuhausJ-U Stolzenburg
Aug 16, 2008·Analytical and Bioanalytical Chemistry·Jochen MetzgerHarald Mischak
Oct 12, 2012·Analytical and Bioanalytical Chemistry·J Giner Martínez-SierraJ I García Alonso
Jan 15, 2013·Analytical and Bioanalytical Chemistry·Ibon IloroFelix Elortza
Jul 11, 2007·World Journal of Urology·Bruce A JulianJan Novak
Aug 19, 2007·World Journal of Urology·Stephane DecramerJoost P Schanstra
Aug 11, 2007·World Journal of Urology·Eric Schiffer
Aug 19, 2007·World Journal of Urology·Dan Theodorescu, Harald Mischak
Jan 5, 2008·World Journal of Urology·Eric SchifferAntonia Vlahou
Jul 16, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Cécile CaubetJoost P Schanstra
Jun 30, 2012·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Katarzyna Taranta-JanuszMarlena Waszkiewicz-Stojda
Jul 9, 2011·Clinical and Experimental Medicine·Juan YangChen Huang
Apr 2, 2011·Science China. Life Sciences·Chen ShaoYouHe Gao
Mar 13, 2013·Molecular Diagnosis & Therapy·Steve GoodisonVirginia Urquidi
Jul 9, 2013·Biochimica Et Biophysica Acta·Eva Rodríguez-SuárezHarald Mischak
Jan 1, 2014·Journal of Proteomics·Maria FrantziAxel S Merseburger
Jul 10, 2010·Molecular & Cellular Proteomics : MCP·David M GoodPhilippe Schmitt-Kopplin
Jul 30, 2011·Molecular & Cellular Proteomics : MCP·Zhenzhen HuangWei Hang
Jan 10, 2009·Diabetes Technology & Therapeutics·Janet K Snell-BergeonHarald Mischak
Jul 14, 2012·European Heart Journal·Tatiana KuznetsovaJan A Staessen
Feb 20, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jens DrubeJochen H H Ehrich
Aug 1, 2009·Current Opinion in Organ Transplantation·Minnie M Sarwal
Apr 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Na YangSteven Goodison
Jan 7, 2011·BMC Bioinformatics·Mohammed DaknaMark Girolami
Jul 19, 2006·Diagnostic Pathology·Shaheen Emmanuel Lakhan
Oct 27, 2010·PloS One·Alaa AlkhalafUNKNOWN PREDICTIONS Group
May 23, 2012·PloS One·Jesse DawsonWilliam Mullen
May 22, 2013·PloS One·Ángel ArgilésHarald Mischak

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