Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis

The Journal of Organic Chemistry
Abraham J Waldman, Emily P Balskus

Abstract

The molecular architectures and potent bioactivities of diazo-containing natural products have attracted the interest of synthetic and biological chemists. Despite this attention, the biosynthetic enzymes involved in diazo group construction have not been identified. Here, we show that the ATP-dependent enzyme CreM installs the diazo group in cremeomycin via late-stage N-N bond formation using nitrite. This finding should inspire efforts to use diazo-forming enzymes in biocatalysis and synthetic biology as well as enable genome-based discovery of new diazo-containing metabolites.

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Apr 25, 2019·Bioscience, Biotechnology, and Biochemistry·Yohei Katsuyama
Sep 5, 2019·Chembiochem : a European Journal of Chemical Biology·Guiyun ZhaoYi-Ling Du
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