Discovery of a novel canine respiratory coronavirus support genetic recombination among betacoronavirus1

Virus Research
Shuai LuWenjie Tan

Abstract

Although canine respiratory coronavirus (CRCoV) is an important respiratory pathogen that is prevalent in many countries, only one complete genome sequence of CRCoV (South Korea strain K37) has been obtained to date. Genome-wide analyses and recombination have rarely been conducted, as small numbers of samples and limited genomic characterization have previously prevented further analyses. Herein, we report a unique CRCoV strain, denoted strain BJ232, derived from a CRCoV-positive dog with a mild respiratory infection. Phylogenetic analysis based on complete genome of all available coronaviruses consistently show that CRCoV BJ232 is most closely related to human coronavirus OC43 (HCoV-OC43) and BCoV, forming a separate clade that split off early from other Betacoronavirus 1. Based on the phylogenetic and SimPlot analysis we propose that CRCoV-K37 was derived from genetic recombination between CRCoV-BJ232 and BCoV. In detail, spike (S) gene of CRCoV-K37 clustered with CRCoV-BJ232. However orf1ab, membrane (M) and nucleocapsid (N) genes were more related to Bovine coronavirus (BCoV) than CRCoV-B232. Molecular epidemic analysis confirmed the prevalence of CRCoV-BJ232 lineage around the world for a long time. Recombinant events amo...Continue Reading

Citations

Oct 17, 2017·International Journal of Molecular Sciences·Susanna K P LauPatrick C Y Woo
Jul 8, 2020·Pathogens·Ahmed S Abdel-Moneim, Elsayed M Abdelwhab
Sep 25, 2020·Veterinary Medicine and Science·Md Mijanur RahmanRekha Akter
Apr 15, 2018·Scientific Reports·Anthony ChamingsSoren Alexandersen
Feb 11, 2021·Life·Paolo Bonilauri, Gianluca Rugna
Feb 4, 2021·Voprosy virusologii·S B YatsyshinaV G Akimkin
Mar 9, 2021·Biology Bulletin of the Russian Academy of Sciences·I M DonnikA M Ermakov
Aug 29, 2021·Viruses·Chao-Nan LinPeck Toung Ooi

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Methods Mentioned

BETA
reverse transcription-PCR
PCR

Software Mentioned

PhyML
SimPlot
MAFFT

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